BRENDA - Enzyme Database

Purification and characterization of beta-methylaspartase from Fusobacterium varium

Bearne, S.L.; White, R.L.; MacDonnell, J.E.; Bahrami, S.; Gronlund, J.; Mol. Cell. Biochem. 221, 117-126 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.1.2
NH4+
approx. 65% of K+ activation
Fusobacterium varium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
0.51
-
(2S,3S)-methylaspartate
25°C, pH 9.7
Fusobacterium varium
4.3.1.2
1.5
-
(S)-aspartate
25°C, pH 9.7
Fusobacterium varium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
approx. 5% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Cu2+
approx. 10% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Fe2+
approx. 5% of Mg2+ activation
Fusobacterium varium
4.3.1.2
K+
required for activity
Fusobacterium varium
4.3.1.2
Li+
approx. 20% of K+ activation
Fusobacterium varium
4.3.1.2
Mg2+
required for activity
Fusobacterium varium
4.3.1.2
Mn2+
approx. 40% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Na+
approx. 7% of K+ activation
Fusobacterium varium
4.3.1.2
Ni2+
approx. 10% of Mg2+ activation
Fusobacterium varium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
46000
-
2 * 46000, SDS-PAGE
Fusobacterium varium
4.3.1.2
88000
-
gel filtration
Fusobacterium varium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Fusobacterium varium
-
mesaconate + NH3
-
Fusobacterium varium
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Fusobacterium varium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
heat, ammonium sulfate, gel filtration, DEAE-Sepharose, ethanol
Fusobacterium varium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
137
-
-
Fusobacterium varium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(S)-aspartate
-
653208
Fusobacterium varium
fumarate + NH3
-
653208
Fusobacterium varium
?
4.3.1.2
L-threo-3-methylaspartate
-
653208
Fusobacterium varium
mesaconate + NH3
-
653208
Fusobacterium varium
r
4.3.1.2
NH3 + (2E)-2-methylbut-2-enedioate
-
653208
Fusobacterium varium
(2S,3S)-3-methylaspartate
major product
653208
Fusobacterium varium
r
4.3.1.2
NH3 + (2E)-2-methylbut-2-enedioate
-
653208
Fusobacterium varium
(2S,3R)-3-methylaspartate
minor product
653208
Fusobacterium varium
r
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 46000, SDS-PAGE
Fusobacterium varium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
9.5
-
amination
Fusobacterium varium
4.3.1.2
9.7
-
deamination
Fusobacterium varium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.1.2
8.5
11
55% of maximal amination activity at pH 8.5, 75% of maximal amination activity at pH 11.0, 30% of maximal deamination activity at pH 8.5, 15% of maximal deamination activity at pH 11.0
Fusobacterium varium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.1.2
NH4+
approx. 65% of K+ activation
Fusobacterium varium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.2
0.51
-
(2S,3S)-methylaspartate
25°C, pH 9.7
Fusobacterium varium
4.3.1.2
1.5
-
(S)-aspartate
25°C, pH 9.7
Fusobacterium varium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
approx. 5% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Cu2+
approx. 10% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Fe2+
approx. 5% of Mg2+ activation
Fusobacterium varium
4.3.1.2
K+
required for activity
Fusobacterium varium
4.3.1.2
Li+
approx. 20% of K+ activation
Fusobacterium varium
4.3.1.2
Mg2+
required for activity
Fusobacterium varium
4.3.1.2
Mn2+
approx. 40% of Mg2+ activation
Fusobacterium varium
4.3.1.2
Na+
approx. 7% of K+ activation
Fusobacterium varium
4.3.1.2
Ni2+
approx. 10% of Mg2+ activation
Fusobacterium varium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
46000
-
2 * 46000, SDS-PAGE
Fusobacterium varium
4.3.1.2
88000
-
gel filtration
Fusobacterium varium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Fusobacterium varium
-
mesaconate + NH3
-
Fusobacterium varium
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
heat, ammonium sulfate, gel filtration, DEAE-Sepharose, ethanol
Fusobacterium varium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
137
-
-
Fusobacterium varium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(S)-aspartate
-
653208
Fusobacterium varium
fumarate + NH3
-
653208
Fusobacterium varium
?
4.3.1.2
L-threo-3-methylaspartate
-
653208
Fusobacterium varium
mesaconate + NH3
-
653208
Fusobacterium varium
r
4.3.1.2
NH3 + (2E)-2-methylbut-2-enedioate
-
653208
Fusobacterium varium
(2S,3S)-3-methylaspartate
major product
653208
Fusobacterium varium
r
4.3.1.2
NH3 + (2E)-2-methylbut-2-enedioate
-
653208
Fusobacterium varium
(2S,3R)-3-methylaspartate
minor product
653208
Fusobacterium varium
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 46000, SDS-PAGE
Fusobacterium varium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
9.5
-
amination
Fusobacterium varium
4.3.1.2
9.7
-
deamination
Fusobacterium varium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.1.2
8.5
11
55% of maximal amination activity at pH 8.5, 75% of maximal amination activity at pH 11.0, 30% of maximal deamination activity at pH 8.5, 15% of maximal deamination activity at pH 11.0
Fusobacterium varium