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Literature summary extracted from

  • Iwamoto, Y.; Iriyama, K.; Osatomi, K.; Oda, T.; Muramatsu, T.
    Primary structure and chemical modification of some amino acid residues of bifunctional alginate lyase from a marine bacterium Pseudoalteromonas sp. strain no. 272 (2002), J. Protein Chem., 21, 455-463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis Pseudoalteromonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate reacts with Asp and Glu residues, causes a slight decrease in activity Pseudoalteromonas sp.
4.2.2.3 2,4,6-Trinitrobenzene sulfonate reacts with Lys residues, causes a slight decrease in activity Pseudoalteromonas sp.
4.2.2.3 Butane-2,3-dione reacts with Arg residues, causes a slight decrease in activity Pseudoalteromonas sp.
4.2.2.3 N-bromosuccimide complete loss of activity, reacts with Trp residues Pseudoalteromonas sp.
4.2.2.3 Tetranitromethane reacts with Tyr residues, causes a slight decrease in activity Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information kinetics Pseudoalteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 25550
-
 amino acid sequence determination Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudoalteromonas sp.
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-
4.2.2.3 Pseudoalteromonas sp. 272
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 poly-alpha1,4-L-guluronic acid
-
 Pseudoalteromonas sp. alpha-D-glucuronate + ?
-
 ?
4.2.2.3 poly-alpha1,4-L-guluronic acid
-
 Pseudoalteromonas sp. 272 alpha-D-glucuronate + ?
-
 ?
4.2.2.3 poly-beta1,4-D-mannuronate
-
 Pseudoalteromonas sp. beta-D-mannuronate
-
 ?
4.2.2.3 poly-beta1,4-D-mannuronate
-
 Pseudoalteromonas sp. 272 beta-D-mannuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 AAlyase
-
 Pseudoalteromonas sp.
4.2.2.3 alginate lyase
-
 Pseudoalteromonas sp.