Any feedback?
Literature summary extracted from
- Bovine pancreatic elastase II cleaves Gln-Ile bond (2001), J. Protein Chem., 20, 577-584.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.71 | chymostatin | - |
Bos taurus |  |
| 3.4.21.71 | Cu2+ | 1 mM, 50% inhibition | Bos taurus | |
| 3.4.21.71 | Elastinal | - |
Bos taurus | |
| 3.4.21.71 | Hg2+ | 1 mM, 50% inhibition | Bos taurus | |
| 3.4.21.71 | leupeptin | - |
Bos taurus | |
| 3.4.21.71 | additional information | TLCK and TPCK have only a small effect on the activity | Bos taurus | |
| 3.4.21.71 | NCDC | - |
Bos taurus | |
| 3.4.21.71 | phenylmethylsulfonyl fluoride | completely inhibited | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.71 | 0.0083 | - |
Gly-Ile-Asp-Val-Gln-Ile-Tyr | pH 8.0, 25°C | Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.71 | additional information | most metal ions have almost no effect on the activity | Bos taurus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.71 | 25000 | - |
gel filtration | Bos taurus |
| 3.4.21.71 | 29000 | - |
SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.71 | Bos taurus | - |
bovine | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.71 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.71 | 4.76 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.71 | Gly-Ile-Asp-Val-Gln-Ile-Tyr + H2O | sequence in phenylalanine oxidase | Bos taurus | Gly-Ile-Asp-Val-Gln + Ile-Tyr + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.71 | monomer | 1 * 29000, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.71 | bovine pancreatic elastase | - |
Bos taurus |
| 3.4.21.71 | GICP | - |
Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.71 | 40 | 50 | incubation of purified enzyme for 1 h causes a slight loss of activity, less than 10% | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.71 | 2.1 | - |
Gly-Ile-Asp-Val-Gln-Ile-Tyr | pH 8.0, 25°C | Bos taurus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.71 | 8 | 9 | - |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
