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Literature summary extracted from

  • Ahsan, M.N.; Watabe, S.
    Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus (2001), J. Protein Chem., 20, 49-58.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 2-mercaptoethanol 0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity Engraulis japonicus
3.4.21.4 antipain 0.01 mM, isoenzyme aT-I loses 96% of its activity, isoenzyme aT-II loses 99% of its activity Engraulis japonicus
3.4.21.4 benzamidine 1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity Engraulis japonicus
3.4.21.4 leupeptin 0.1 mM, isoenzyme aT-I loses 97% of its activity, isoenzyme aT-II completely loses its activity Engraulis japonicus
3.4.21.4 PMSF 0.1 mM, isoenzyme aT-I loses 89% of its activity, isoenzyme aT-II loses 80% of its activity Engraulis japonicus
3.4.21.4 Soybean trypsin inhibitor 0.1 mg/ml, isoenzyme aT-I loses 95% of its activity, isoenzyme aT-II loses 97% of its activity Engraulis japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.014
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
3.4.21.4 0.031
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
3.4.21.4 0.033
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
3.4.21.4 0.037
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 24000
-
isoenzyms aT-I and aT-II, gel filtration Engraulis japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Engraulis japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 two isoforms: aT-I and aT-II Engraulis japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 intestine visceral extract consisting mainly of pyloric ceca and intestine Engraulis japonicus
-
3.4.21.4 pyloric cecum visceral extract consisting mainly of pyloric ceca and intestine Engraulis japonicus
-
3.4.21.4 viscus consisting mainly of pyloric ceca and intestine Engraulis japonicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 4.85
-
aT-I Engraulis japonicus
3.4.21.4 9.26
-
aT-II Engraulis japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 benzoyl-Arg 4-nitroanilide + H2O
-
Engraulis japonicus benzoyl-Arg + 4-nitroaniline
-
?
3.4.21.4 benzoyl-Arg ethyl ester + H2O
-
Engraulis japonicus benzoyl-Arg + ethanol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 aT-I
-
Engraulis japonicus
3.4.21.4 aT-II
-
Engraulis japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 60
-
isoform aT-I and aT-II Engraulis japonicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.4 40 65 40°C: about 30% of maximal activity, 65°C: about 90% of maximal activity, isoenzyme aT-I. 40°C: about 30% of maximal activity, 65°C: about 70% of maximal activity, isoenzyme aT-II Engraulis japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 10
-
30 min, stable, isoenzyme aT-I and aT-II Engraulis japonicus
3.4.21.4 20
-
30 min, isoenzyme aT-I loses about 20% of its activity, isoenzyme aT-II loses about 10% of its activity Engraulis japonicus
3.4.21.4 30
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
3.4.21.4 40
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
3.4.21.4 50
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
3.4.21.4 60
-
30 min, isoenzyme aT-I loses about 50% of its activity, isoenzyme aT-II loses about 55% of its activity Engraulis japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 0.3
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
3.4.21.4 1.94
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
3.4.21.4 2.94
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
3.4.21.4 3.7
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
3.4.21.4 42.9
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 9.5
-
isoform aT-I and aT-II Engraulis japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 7 12 pH 7.0: about 65% of maximal activity, pH 12: about 90% of maximal activity, isoenzyme aT-I. pH 7.0: about 75% of maximal activity, pH 12: about 80% of maximal activity, isoenzyme aT-II Engraulis japonicus