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Literature summary extracted from

  • Harrach, T.; Eckert, K.; Maurer, H.R.; Machleidt, I.; Machleidt, W.; Nuck, R.
    Isolation and characterization of two forms of an acidic bromelain stem proteinase (1998), J. Protein Chem., 17, 351-361.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 cystatin chicken, weak inhibition Ananas comosus
3.4.22.32 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E-64, slow inactivation Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.003
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.0039
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.0074
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.0081
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.0554
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.0636
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 23550
-
 isozyme SBA7A, mass spectroscopy Ananas comosus
3.4.22.32 23560
-
 isozyme SBA/b, mass spectroscopy Ananas comosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.32 protein + H2O Ananas comosus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
 2 isozymes SBA/a and SBA/b
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.4.22.32 oxidation of the active site thiol leads to the corresponding sulfinic acid, which is catalytically inactive, irreversible oxidative degradation may lead to reduced activity of isozyme SBA/b with substrate Pyr-Phe-Lys-4-nitroanilide Ananas comosus

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.32 glycoprotein both isozymes are highly glycosylated, isozyme SBA/a is about 2fold more glycosylated than isozyme SBA/b, glycoslyation composition Ananas comosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.32 2 isozymes SBA/a and SBA/b Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem crude bromelain extract Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.32 0.17
-
 partially purified enzyme, 37°C, pH 6.5 Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
3.4.22.32 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
3.4.22.32 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
3.4.22.32 protein + H2O
-
 Ananas comosus peptides
-
 ?
3.4.22.32 Pyr-Phe-Lys-4-nitroanilide + H2O isozyme SBA/b is about 3fold less active than isozyme SBA/a Ananas comosus Pyr-Phe-Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.32 monomer 1 * 23550-27900, isozyme SBA/a, mass spectroscopy and SDS-PAGE, 1 * 23560-27800, isozyme SBA/b, mass spectroscopy and SDS-PAGE Ananas comosus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 acidic bromelain stem proteinase
-
 Ananas comosus
3.4.22.32 Pineapple stem bromelain
-
 Ananas comosus
3.4.22.32 SBA
-
 Ananas comosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.024
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.04
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.49
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 0.56
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
3.4.22.32 5.1
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
3.4.22.32 6.08
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
3.4.22.32 6.4
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 6 7 substrate-dependent Ananas comosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.32 2 8
-
 Ananas comosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.32 additional information
-
 additional information k2/Ki for E-64 Ananas comosus
3.4.22.32 0.1
-
 chicken cystatin both isozymes, pH 6.0, 25°C Ananas comosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.32 Ananas comosus isoelectric focusing, isozyme SBA/b, and isozyme SBA/a variant 2
-
 4.8
3.4.22.32 Ananas comosus isoelectric focusing, isozyme SBA/a variant 1
-
 4.9