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Literature summary extracted from

  • Altamirano, C.V.; Bartels, C.F.; Lockridge, O.
    The butyrylcholinesterase K-variant shows similar cellular protein turnover and quaternary interaction to the wild-type enzyme (2000), J. Neurochem., 74, 869-877.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8 expressed in CHO cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 A539T K-variant Homo sapiens
3.1.1.8 E497V/A539T J-variant Homo sapiens
3.1.1.8 E797V catalytic activity similar to wild-type enzyme Homo sapiens

General Stability

EC Number General Stability Organism
3.1.1.8 half-life of wild-type enzyme in culture: 1.41h, half-life of A539T mutant enzyme in culture: 1.55h, half-life of E497V/A539T mutant enzyme in culture: 1.49h Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.008
-
 Benzoylcholine wild-type and A539T mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.009
-
 Benzoylcholine E497V/A539T mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.011
-
 Benzoylcholine E497V mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.017
-
 butyrylthiocholine A539T mutant, E497V/A539T mutant and E497V mutant, pH 7, 25°C Homo sapiens
3.1.1.8 0.023
-
 butyrylthiocholine wild-type, pH 7, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 benzoylcholine + H2O
-
 Homo sapiens choline + benzoate
-
 ?
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.8 tetramer in the presence of poly-L-proline Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 223
-
 Benzoylcholine E497V/A539T mutant, pH 7, 25°C Homo sapiens
3.1.1.8 242
-
 Benzoylcholine wild-type, pH 7, 25°C Homo sapiens
3.1.1.8 265
-
 Benzoylcholine A539T mutant, pH 7, 25°C Homo sapiens
3.1.1.8 303
-
 Benzoylcholine E497V mutant, pH 7, 25°C Homo sapiens
3.1.1.8 508
-
 butyrylthiocholine E497V/A539T mutant, pH 7, 25°C Homo sapiens
3.1.1.8 542
-
 butyrylthiocholine A539T mutant and E497V mutant, pH 7, 25°C Homo sapiens
3.1.1.8 565
-
 butyrylthiocholine wild-type, pH 7, 25°C Homo sapiens