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Literature summary extracted from

  • Layh, N.; Parratt, J.; Willetts, A.
    Characterization and partial purification of an enantioselective arylacetonitrilase from Pseudomonas fluorescens DSM 7155 (1998), J. Mol. Catal. B, 5, 467-474.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.5 2-mercaptoethanol 1.4fold at 50 mM, 1.6fold activation at 100 mM Pseudomonas fluorescens
3.5.5.5 phenylacetonitrile absolutely required as growth nitrogen source substrate for induction of the enzyme, cannot be substituted by ammonia or epsilon-caprolactam Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.5 Ag+ complete inhibition at 1 mM Pseudomonas fluorescens
3.5.5.5 Cu2+ complete inhibition at 1 mM Pseudomonas fluorescens
3.5.5.5 Fe2+ complete inhibition at 10 mM Pseudomonas fluorescens
3.5.5.5 Fe3+ complete inhibition at 1 mM Pseudomonas fluorescens
3.5.5.5 additional information no inhibition by KCN, EDTA, D-cycloserine, and Mn2+ Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.5 0.065
-
 phenylacetonitrile pH 7.4, 30°C Pseudomonas fluorescens
3.5.5.5 0.087
-
 rac 2-(methoxy)-mandelonitrile pH 7.4, 30°C Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.5 additional information enzyme does not seem to require metals for activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.5 13000
-
 gel filtration Pseudomonas fluorescens
3.5.5.5 38000
-
 1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE Pseudomonas fluorescens
3.5.5.5 40000
-
 1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE Pseudomonas fluorescens
3.5.5.5 57000
-
 1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas fluorescens
-
 DSM 7155, inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.5 partial, 259fold Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.5 90
-
 partially purified enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 2-phenylpropionitrile + H2O low activity, 2% activity compared to phenylacetonitrile Pseudomonas fluorescens 2-phenylpropionate + NH3
-
 ?
3.5.5.5 additional information no activity with benzonitrile, 2-cyanotoluene, 3-cyanotoluene, 3-cyanobenzothiophene, propionitrile, butyronitrile, and valeronitrile, 2-phenylbutyronitrile is a poor substrate, enzyme is enantioselective and specific for arylacetonitriles Pseudomonas fluorescens ?
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O best substrate Pseudomonas fluorescens phenylacetate + NH3
-
 ?
3.5.5.5 rac 2-(methoxy)-mandelonitrile + H2O
-
 Pseudomonas fluorescens (R)-2-(methoxy)-mandelic acid + (S)-2-(methoxy)-mandelic acid + NH3 10% activity compared to phenylacetonitrile, 92% of the product is the (R)-enantiomer when the overall conversion rate is 50%, at 85% overall conversion, the level of formed (R)-enantiomer is 27% ?

Subunits

EC Number Subunits Comment Organism
3.5.5.5 trimer 1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.5.5.5 nitrilase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.5 55
-
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.5 9
-
-
 Pseudomonas fluorescens