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Literature summary extracted from

  • Kulik, V.; Weyand, M.; Seidel, R.; Niks, D.; Arac, D.; Dunn, M.F.; Schlichting, I.
    On the role of alphaThr183 in the allosteric regulation and catalytic mechanism of tryptophan synthase (2002), J. Mol. Biol., 324, 677-690.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.20 crystallization of wild-type enzyme alone and complexed with L-serine and 3-indolylpropanol 3'-phosphate, and of mutant T183V complexed with L-serine, 1-(indol-3-yl)glycerol 3-phosphate, and 3-indolylpropanol 3'-phosphate, hanging drop method in the dark at room temperature, equal volumes of protein solution, containing 10 mg/ml protein, 50 mM Na-bicine, pH 7.8, 10 mM Na-EDTA, 1 mM dithioerythritol, 0.02 mM pyridoxal 5'-phosphate, and of reservoir solution, containing 50 mM Na-bicine, pH 7.8, 5 mM dithioerythritol, 5 mM Na-EDTA, 0.1 mM pyridoxal 5'-phosphate, 2 mM spermine, 8-12% w/v PEG 8000, X-ray diffraction structure determination and analysis at different resolutions 1.45-2.3 A, comparison of crystal structures of the different complexes Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 T183V site-directed mutagenesis, isosteric alpha-subunit mutant, exchange of a highly conserved residue, strongly impaired allosteric alpha-beta communication, 100fold reduced alpha-reaction activity, beta-reaction activity is not affected, effects are due to a missing hydrogen bind between alphaT183 and the catalytic residue alphaAsp60 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.20 DL-glycerol 3-phosphate inhibits the reaction of indole with the enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism of alpha- and beta-reaction step, T183 and D60 are involved Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.20 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bifunctional enzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bifunctional enzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indoline
-
 Salmonella enterica subsp. enterica serovar Typhimurium dihydro-iso-L-tryptophan + H2O i.e. DIT ?
4.2.1.20 additional information allostery and substrate channeling, wild-type and mutant T183V Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 TRPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.00017
-
 L-serine overall-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.00017
-
 1-(indol-3-yl)glycerol 3-phosphate overall-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.00059
-
 1-(indol-3-yl)glycerol 3-phosphate alpha-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.063
-
 1-(indol-3-yl)glycerol 3-phosphate alpha-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 2.61
-
 L-serine overall-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 2.61
-
 1-(indol-3-yl)glycerol 3-phosphate overall-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3 6 L-serine overall-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3 6 1-(indol-3-yl)glycerol 3-phosphate overall-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3.3
-
 L-serine beta-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3.3
-
 indole beta-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3.3
-
 L-serine beta-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3.3
-
 indole beta-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 4.51
-
 L-serine beta-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 4.51
-
 indole beta-reaction, pH 7.8, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 5.24
-
 L-serine beta-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 5.24
-
 indole beta-reaction, pH 7.8, 25°C, mutant T183V enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate dependent on, bound to the enzyme Salmonella enterica subsp. enterica serovar Typhimurium