Literature summary extracted from
Kaushik, J.K.; Ogasahara, K.; Yutani, K.
The unusually slow relaxation kinetics of the folding-unfolding of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus (2002), J. Mol. Biol., 316, 991-1003.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.19.3 |
C142S/C188S |
small thermodynamic stability of the mutant enzyme C142S/C188S at low pH. The mutant enzyme is monomeric below pH 2.7, dimeric around pH 3 and tetrameric above pH 4.5. The heat-denaturation of the mutant enzyme is completely reversible at pH 2.3, although the unfolding-refolding reactions are characterized by extremely slow kinetics |
Pyrococcus furiosus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.19.3 |
Pyrococcus furiosus |
- |
- |
- |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
3.4.19.3 |
the heat-denaturation of the mutant enzyme C142S/C188S is completely reversible at pH 2.3, although the unfolding-refolding reactions are characterized by extremely slow kinetics |
Pyrococcus furiosus |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.19.3 |
dimer |
the mutant enzyme C142S/C188S is monomeric below pH 2.7, dimeric around pH 3 and tetrameric above pH 4.5 |
Pyrococcus furiosus |
3.4.19.3 |
monomer |
the mutant enzyme C142S/C188S is monomeric below pH 2.7, dimeric around pH 3 and tetrameric above pH 4.5 |
Pyrococcus furiosus |
3.4.19.3 |
tetramer |
the mutant enzyme C142S/C188S is monomeric below pH 2.7, dimeric around pH 3 and tetrameric above pH 4.5 |
Pyrococcus furiosus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.19.3 |
pyrrolidone carboxyl peptidase |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.4.19.3 |
47 |
- |
thermodynamics of heat denaturation of the monomeric enzyme form of mutant enzyme C142S/C188S at pH 2.3. The mechanism of refolding is a two-state process. The equilibrium establishes with a relaxation time of 5080 s at Tm = 46.5°C |
Pyrococcus furiosus |