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Literature summary extracted from

  • Yoon, H.J.; Mikami, B.; Hashimoto, W.; Murata, K.
    Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution (1999), J. Mol. Biol., 290, 505-514.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.3 hanging drop vapour diffusion method, 0.003 ml protein solution: 17.2 mg/ml protein, 0.1 M HEPES, pH 7.5, + 0.003 ml bottom solution: 47-49% saturated ammonium sulfate, 0.1 M HEPES, pH 7.5, 20°C, 1 month, for large crystals micro- and macroseeding is used, crystal are soaked in heavy-atom derivatives for 1-4 h for X-ray diffraction structure determination and analysis at 1.78-10.0 A resolution Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 sulfate sulfate binding site structure Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Sphingomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 poly-beta-D-mannuronate i.e. alginate, cleavage site structure Sphingomonas sp. oligomannuronate
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 More the enzyme shows an (alpha6/alpha5)-barrel structure with a tunnel-like active site cleft Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase1-III
-
Sphingomonas sp.
4.2.2.3 ALYIII
-
Sphingomonas sp.