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Literature summary extracted from

  • Azarkan, M.; El Moussaoui, A.; van Wuytswinkel, D.; Dehon, G.; Looze, Y.
    Fractionation and purification of the enzymes stored in the latex of Carica papaya (2003), J. Chromatogr. B, 790, 229-238.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.30
-
 Carica papaya

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.25 extracellular
-
 Carica papaya
-
-
3.4.22.30 extracellular
-
 Carica papaya
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.30 23500
-
-
 Carica papaya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.25 protein + H2O Carica papaya
-
 peptides
-
 ?
3.4.22.30 protein + H2O Carica papaya
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.6 Carica papaya
-
-
-
3.4.22.25 Carica papaya
-
-
-
3.4.22.30 Carica papaya
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.6 proteolytic modification the enzyme is synthesized as inactive precursor that converts into mature enzyme within 2 min after wounding the plant when the latex is abruptly expelled Carica papaya
3.4.22.25 additional information enzyme is synthesized as inactive proenzyme, and rapidly converted to the active from within 2 min after wounding of the plant Carica papaya
3.4.22.30 additional information enzyme is synthesized as inactive proenzyme, and rapidly converted to the active from within 2 min after wounding of the plant Carica papaya

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.6 supports: phenyl-Hg+ CH3COO- and glutathione-S-S-(2-pyridyl), further purification through the S-thiol poly(ethylene glycol) derivatization technique Carica papaya
3.4.22.25 several ligands possible for affinity chromatography are presented, fractionation on hydrophobic and cation-exchange supports, overview Carica papaya
3.4.22.30 to homogeneity, several ligands possible for affinity chromatography are presented, fractionation on hydrophobic and cation-exchange supports, overview Carica papaya

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.25 preferential cleavage: Gly-/-, in proteins and small molecule substrates specific for Gly at posotion P1 due to an exchange conserved residues Gly23 to Glu and Gly65 to Arg, 2 free thiol functions of cysteinyl residues are essential for the catalytic competence of the enzyme Carica papaya
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.6 latex
-
 Carica papaya
-
3.4.22.25 latex
-
 Carica papaya
-
3.4.22.30 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.25 protein + H2O
-
 Carica papaya peptides
-
 ?
3.4.22.30 protein + H2O
-
 Carica papaya peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.25 Glycyl endopeptidase
-
 Carica papaya