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Literature summary extracted from

  • Wenzel, N.F.; Carenbauer, A.L.; Pfiester, M.P.; Schilling, O.; Meyer-Klaucke, W.; Makaroff, C.A.; Crowder, M.W.
    The binding of iron and zinc to glyoxalase II occurs exclusively as di-metal centers and is unique within the metallo-beta-lactamase family (2004), J. Biol. Inorg. Chem., 9, 429-438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 overexpression in Escherichia coli in minimal medium Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.139
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium without in presence of zinc Arabidopsis thaliana
3.1.2.6 0.14
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, medium with Zn added, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.209
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium without added metal ion Arabidopsis thaliana
3.1.2.6 0.21
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, minimal medium, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.6
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, medium with Fe added, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.6
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium in presence of iron Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.6 cytosol
-
 Arabidopsis thaliana 5829
-
3.1.2.6 cytosol cytosolic isozyme Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+
-
 Arabidopsis thaliana
3.1.2.6 Iron 0.55 mol per mol of protein in minimal medium, located in the dinuclear site, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium Arabidopsis thaliana
3.1.2.6 Mn2+
-
 Arabidopsis thaliana
3.1.2.6 Mn2+ 0.05 mol per mol of protein in minimal medium, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium Arabidopsis thaliana
3.1.2.6 additional information metal binding structure, and metal content under different media conditions, overview, enzyme shows positive cooperative metal binding Arabidopsis thaliana
3.1.2.6 additional information overexpresssion of enzyme in minmal media containing either Zn, Fe, or Mn results in enzyme binding an average of 1 equivalent of metal ion, and presence of antiferomagnetically and ferromagnetically coupled dinuclear metal centers. Enzyme does not exist as a mononuclear metal ion containing enzyme, and shows positive cooperativity in metal binding Arabidopsis thaliana
3.1.2.6 Zn2+
-
 Arabidopsis thaliana
3.1.2.6 Zn2+ 0.5 mol per mol of protein in minimal medium, located in the dinuclear site, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Arabidopsis thaliana
-
 cytoslic isozyme
-
3.1.2.6 Arabidopsis thaliana
-
 overexpresssion in minmal media containing either Zn, Fe, or Mn
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Arabidopsis thaliana glutathione + D-lactate
-
 ?
3.1.2.6 S-lactoylglutathione + H2O
-
 Arabidopsis thaliana D-lactate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Arabidopsis thaliana
3.1.2.6 Germ cell specific protein
-
 Arabidopsis thaliana
3.1.2.6 GLO II
-
 Arabidopsis thaliana
3.1.2.6 GLX2-2
-
 Arabidopsis thaliana
3.1.2.6 glyoxalase 2
-
 Arabidopsis thaliana
3.1.2.6 glyoxalase II
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Arabidopsis thaliana
3.1.2.6 More the enzyme belongs to the metallo-beta-lactamase superfamily Arabidopsis thaliana
3.1.2.6 Round spermatid protein RSP29
-
 Arabidopsis thaliana
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 136
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, medium with Fe added, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 136
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium in presence of iron Arabidopsis thaliana
3.1.2.6 240
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, medium with Zn added, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 240
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium without in presence of zinc Arabidopsis thaliana
3.1.2.6 470
-
 S-D-lactoylglutathione recombinant cytosolic isozyme, minimal medium, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 470
-
 S-Lactoylglutathione pH 7.2, 25°C, growth on minimal medium without added metal ion Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
 assay at Arabidopsis thaliana