Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.; Yakunin, A.F.; Kuznetsova, E.; Xu, X.; Pennycooke, M.; Gu, J.; Cheung, F.; Proudfoot, M.; Arrowsmith, C.H.; Joachimiak, A.; Edwards, A.M.; Christendat, D.
    Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum (2004), J. Biol. Chem., 279, 517-526.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.18 Cl- activates at chloride ion concentration up to 50 mM Thermoplasma acidophilum
3.1.3.18 KCl below 50 mM Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.18
-
 Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.18 X-ray analysis Thermoplasma acidophilum

General Stability

EC Number General Stability Organism
3.1.3.18 stable Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.18 Ca2+ inhibits Thermoplasma acidophilum
3.1.3.18 Cl- inhibits at high concentration, above 50 mM, competitive inhibition; total inhibition at chloride ion concentration of 600 mM Thermoplasma acidophilum
3.1.3.18 KCl total inhibition at chloride ion concentration of 600 mM Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.18 0.029
-
 2-Phosphoglycolate pH 7.5, 70°C Spinacia oleracea
3.1.3.18 0.037
-
 Phosphoglycolate
-
 Thermoplasma acidophilum
3.1.3.18 0.037
-
 2-Phosphoglycolate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 0.061
-
 2-Phosphoglycolate pH 7.5, 70°C Homo sapiens
3.1.3.18 0.17
-
 diphosphate
-
 Thermoplasma acidophilum
3.1.3.18 0.17
-
 p-nitrophenyl phosphate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 3.1
-
 p-nitrophenyl phosphate
-
 Thermoplasma acidophilum
3.1.3.18 3.1
-
 diphosphate pH 7.5, 70°C Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.18 Cl- activates at low concentration, below 50 Thermoplasma acidophilum
3.1.3.18 Co2+
-
 Thermoplasma acidophilum
3.1.3.18 Co2+ activates Thermoplasma acidophilum
3.1.3.18 Co2+ activates, KD = 0.036 +/- 0.004 Thermoplasma acidophilum
3.1.3.18 KCl activates at chloride ion concentration up to 50 mM Thermoplasma acidophilum
3.1.3.18 Mg2+
-
 Thermoplasma acidophilum
3.1.3.18 Mg2+ activates Thermoplasma acidophilum
3.1.3.18 Mg2+ activates, KD = 0.13 +/- 0.01 Thermoplasma acidophilum
3.1.3.18 Mn2+
-
 Thermoplasma acidophilum
3.1.3.18 Mn2+ activates Thermoplasma acidophilum
3.1.3.18 Mn2+ activates, KD = 0.02 +/- 0.004 Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.18 phosphoglycolate + H2O Thermoplasma acidophilum
-
 glycolate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.18 Homo sapiens
-
-
-
3.1.3.18 Spinacia oleracea
-
-
-
3.1.3.18 Thermoplasma acidophilum Q9HLQ2
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.18
-
 Thermoplasma acidophilum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.18 blood cell red Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.18 0.94
-
 p-nitrophenyl phosphate Thermoplasma acidophilum
3.1.3.18 11.55
-
 diphosphate Thermoplasma acidophilum
3.1.3.18 19.82
-
 substrate phosphoglycolate Thermoplasma acidophilum
3.1.3.18 19.82
-
 phosphoglycolate Thermoplasma acidophilum

Storage Stability

EC Number Storage Stability Organism
3.1.3.18 4°C, 5% glycerol, 0.5 M NaCl, no loss in activity after several months Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.18 2-Phosphoglycolate + H2O
-
 Homo sapiens Glycolate + phosphate
-
 ?
3.1.3.18 2-Phosphoglycolate + H2O
-
 Spinacia oleracea Glycolate + phosphate
-
 ?
3.1.3.18 2-Phosphoglycolate + H2O
-
 Thermoplasma acidophilum Glycolate + phosphate
-
 ?
3.1.3.18 diphosphate + H2O
-
 Thermoplasma acidophilum 2 phosphate
-
 ?
3.1.3.18 p-nitrophenyl phosphate + H2O
-
 Thermoplasma acidophilum p-nitrophenol + phosphate
-
 ?
3.1.3.18 phosphoglycolate + H2O
-
 Thermoplasma acidophilum glycolate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.18 dimer gel filtration Thermoplasma acidophilum
3.1.3.18 homodimer gel filtration Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.18 0.031 0.51 2-Phosphoglycolate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 0.58
-
 p-nitrophenyl phosphate
-
 Thermoplasma acidophilum
3.1.3.18 0.58
-
 diphosphate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 4.8
-
 diphosphate
-
 Thermoplasma acidophilum
3.1.3.18 4.8
-
 p-nitrophenyl phosphate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 6.08
-
 diphosphate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 8.26
-
 Phosphoglycolate
-
 Thermoplasma acidophilum
3.1.3.18 8.26
-
 2-Phosphoglycolate pH 7.5, 70°C Thermoplasma acidophilum
3.1.3.18 19
-
 2-Phosphoglycolate pH 7.5, 70°C Homo sapiens
3.1.3.18 292
-
 2-Phosphoglycolate pH 7.5, 70°C Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.18 7.5 8
-
 Thermoplasma acidophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.18 0.17
-
 Ca2+
-
 Thermoplasma acidophilum
3.1.3.18 18.04
-
 KCl
-
 Thermoplasma acidophilum