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Literature summary extracted from

  • Irsch, T.; Krauth-Siegel, R.L.
    Glyoxalase II of African trypanosomes is trypanothione-dependent (2004), J. Biol. Chem., 279, 22209-22217.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.6 additional information no dependance on ionic strength Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6
-
 Trypanosoma brucei
3.1.2.6 DNA and amino acid sequence determination and analysis, overexpression as N-terminally His-tagged and as free enzyme in Escherichia coli Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 additional information the enzyme is not sensitive against ionic strength Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.086
-
 bis-(lactoyl)trypanothione pH 7.2, 25°C Trypanosoma brucei
3.1.2.6 0.086
-
 bis-(lactoyl)trypanothione recombinant enzyme, pH 7.2, 25°C Trypanosoma brucei
3.1.2.6 0.108
-
 mono-(lactoyl)trypanothione pH 7.2, 25°C Trypanosoma brucei
3.1.2.6 0.108
-
 mono-(lactoyl)trypanothione recombinant enzyme, pH 7.2, 25°C Trypanosoma brucei
3.1.2.6 3
-
 S-D-lactoylglutathione above, recombinant enzyme, pH 7.2, 25°C Trypanosoma brucei
3.1.2.6 3
-
 lactoylglutathione or above, pH 7.2, 25°C Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Cobalt 0.6 mol per mol of protein Trypanosoma brucei
3.1.2.6 Iron 0.42 mol per mol of protein Trypanosoma brucei
3.1.2.6 additional information the enzyme contains the highly conserved metal binding motif THXHXDH, total metal ions per mol of enzyme: 1.5 for the His-tagged enzyme, 0.7-0.9 for the free enzyme Trypanosoma brucei
3.1.2.6 additional information sequence contains the highly conserved metal binding motif THXHXDH Trypanosoma brucei
3.1.2.6 Zinc 0.45 mol per mol of protein Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.6 25000
-
 gel filtration Trypanosoma brucei
3.1.2.6 25000
-
 recombinant enzyme, gel filtration Trypanosoma brucei
3.1.2.6 32000
-
 1 * 32000, recombinant enzyme, SDS-PAGE Trypanosoma brucei
3.1.2.6 32000
-
 1 * 32507, calculated, 1 * 32000, SDS-PAGE Trypanosoma brucei
3.1.2.6 32507
-
 1 * 32507, calculated, 1 * 32000, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O Trypanosoma brucei enzyme is involved in the detoxification of ketoaldehydes such as methylglyoxal, which is mainly a byproduct of glycolysis glutathione + D-lactate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Trypanosoma brucei Q6KF36
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6 recombinant enzyme with and without His-tag Trypanosoma brucei
3.1.2.6 recombinant His-tagged and non-tagged enzyme expressed in Escherichia coli Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 additional information
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 bis-(lactoyl)trypanothione + H2O preferred substrate Trypanosoma brucei trypanothione + D-lactate trypathione is bis(glutathionyl)spermidine ?
3.1.2.6 bis-(lactoyl)trypanothione + H2O
-
 Trypanosoma brucei D-lactate + trypanothione
-
 ?
3.1.2.6 lactoylglutathione + H2O enzyme does not show saturation kinetics up to 5 mM Trypanosoma brucei D-lactate + glutathione
-
 ?
3.1.2.6 mono-(lactoyl)trypanothione + H2O preferred substrate Trypanosoma brucei trypanothione + D-lactate trypathione is bis(glutathionyl)spermidine ?
3.1.2.6 mono-(lactoyl)trypanothione + H2O
-
 Trypanosoma brucei D-lactate + trypanothione
-
 ?
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Trypanosoma brucei glutathione + D-lactate
-
 ?
3.1.2.6 S-D-lactoylglutathione + H2O enzyme is involved in the detoxification of ketoaldehydes such as methylglyoxal, which is mainly a byproduct of glycolysis Trypanosoma brucei glutathione + D-lactate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.6 monomer 1 * 32000, recombinant enzyme, SDS-PAGE Trypanosoma brucei
3.1.2.6 monomer 1 * 32507, calculated, 1 * 32000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Trypanosoma brucei
3.1.2.6 Germ cell specific protein
-
 Trypanosoma brucei
3.1.2.6 GLO II
-
 Trypanosoma brucei
3.1.2.6 glyoxalase II
-
 Trypanosoma brucei
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Trypanosoma brucei
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Trypanosoma brucei
3.1.2.6 Round spermatid protein RSP29
-
 Trypanosoma brucei
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7 8 broad Trypanosoma brucei
3.1.2.6 7 8 broad optimum Trypanosoma brucei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.2.6 Trypanosoma brucei calculated from protein sequence
-
 6
3.1.2.6 Trypanosoma brucei calculated 6.5 6