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Literature summary extracted from

  • Thoden, J.B.; Zhuang, Z.; Dunaway-Mariano, D.; Holden, H.M.
    The structure of 4-hydroxybenzoyl-CoA thioesterase from arthrobacter sp. strain SU (2003), J. Biol. Chem., 278, 43709-43716.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.23 purified recombinant wild-type enzyme with and without bound inhibitor, hanging drop vapour diffusion method, 4°C, 18 mg/ml protein solution containing 10 mM HEPES, pH 7.5, 150 mM KCl, 1 mM 1,4-dithio-D,L-threitol, with or without 1 mM 4-hydroxyphenacyl-CoA, plus precipitant solution containing 17-20% PEG 3400, 100 mM MOPS, pH 7.0, and 200 mM LiCl, X-ray diffraction structure determination at 2.6 A resolution, and analysis Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.23 4-hydroxybenzyl-CoA
-
 Arthrobacter sp.
3.1.2.23 4-hydroxyphenacyl-CoA binding mechanism Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.23 additional information
-
 additional information kinetics Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O Arthrobacter sp. enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O Arthrobacter sp. SU / DSM 20407 enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria 4-Hydroxybenzoate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.23 Arthrobacter sp.
-
-
-
3.1.2.23 Arthrobacter sp. SU / DSM 20407
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA active site structure and ligand binding mechanism, overview Arthrobacter sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Arthrobacter sp. 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria Arthrobacter sp. 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O
-
 Arthrobacter sp. SU / DSM 20407 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.23 4-Hydroxybenzoyl-CoA + H2O enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria Arthrobacter sp. SU / DSM 20407 4-Hydroxybenzoate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.23 tetramer dimer of dimers, structure analysis Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.2.23 Hydrolase, 4-hydroxybenzoyl coenzyme A
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.23 25
-
 assay at Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.23 7.5
-
 assay at Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.23 0.0000003
-
 4-hydroxyphenacyl-CoA pH 7.5, 25°C Arthrobacter sp.
3.1.2.23 0.0006
-
 4-hydroxybenzyl-CoA pH 7.5, 25°C Arthrobacter sp.