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Literature summary extracted from

  • Leech, H.K.; Raux, E.; McLean, K.J.; Munro, A.W.; Robinson, N.J.; Borrelly, G.P.; Malten, M.; Jahn, D.; Rigby, S.E.; Heathcote, P.; Warren, M.J.
    Characterization of the cobaltochelatase CbiXL: evidence for a 4Fe-4S center housed within an MXCXXC motif (2003), J. Biol. Chem., 278, 41900-41907.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.3 cloned and overexpressed in Escherichia coli and Bacillus megaterium Priestia megaterium
4.99.1.3 overexpressed in Escherichia coli Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.99.1.3 C259S site-directed mutagenesis Priestia megaterium
4.99.1.3 C262S site-directed mutagenesis Priestia megaterium
4.99.1.3 M257L site-directed mutagenesis Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.99.1.3 Fe2+ iron-sulfur center Priestia megaterium
4.99.1.3 Fe2+ iron-sulfur center Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.3 35000
-
 SDS-PAGE Priestia megaterium
4.99.1.3 38000
-
 SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+ Priestia megaterium biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ Synechocystis sp. biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ Priestia megaterium DSM 509 biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.3 Priestia megaterium
-
 strain DSM509
-
4.99.1.3 Priestia megaterium DSM 509
-
 strain DSM509
-
4.99.1.3 Synechocystis sp.
-
 strain PCC6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.3
-
 Priestia megaterium
4.99.1.3
-
 Synechocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.99.1.3 0.08
-
 overproduced in Escherichia coli Synechocystis sp.
4.99.1.3 1.4
-
 CbiX6H, overproduced in Escherichia coli Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+
-
 Priestia megaterium cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+
-
 Synechocystis sp. cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Priestia megaterium cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Synechocystis sp. cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+
-
 Priestia megaterium DSM 509 cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Priestia megaterium DSM 509 cobalt-sirohydrochlorin + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.99.1.3 CbiX
-
 Priestia megaterium
4.99.1.3 CbiX
-
 Synechocystis sp.
4.99.1.3 CbiX0H
-
 Priestia megaterium
4.99.1.3 CbiXL
-
 Priestia megaterium
4.99.1.3 CbiXL
-
 Synechocystis sp.
4.99.1.3 CbiXOH
-
 Synechocystis sp.
4.99.1.3 cobaltochelatase
-
 Priestia megaterium
4.99.1.3 cobaltochelatase
-
 Synechocystis sp.