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Literature summary extracted from

  • Yanagisawa, T.; Kawakami, M.
    How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense (2003), J. Biol. Chem., 278, 25887-25894.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.5 gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331 Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.5 additional information no inhibition of isozyme IleRS-R2 by pseudomonic acid, i.e. muciproin Pseudomonas fluorescens
6.1.1.5 pseudomonic acid i.e. muciproin Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.5 0.00125
-
 L-isoleucine pH 7.5, recombinant isozyme IleRS-R1 Pseudomonas fluorescens
6.1.1.5 0.007
-
 ATP pH 7.5, recombinant isozyme IleRS-R1 Pseudomonas fluorescens
6.1.1.5 0.01
-
 ATP pH 7.5, native isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 0.012
-
 L-isoleucine pH 7.5, recombinant isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 0.285
-
 ATP pH 7.5, recombinant isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 0.33
-
 ATP pH 7.5, native isozyme IleRS-R2 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle Pseudomonas fluorescens
-
 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle Pseudomonas fluorescens NCIB 10586
-
 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Pseudomonas fluorescens
-
 gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli
-
6.1.1.5 Pseudomonas fluorescens Q8L1B1 pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2
-
6.1.1.5 Pseudomonas fluorescens NCIB 10586
-
 gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli
-
6.1.1.5 Pseudomonas fluorescens NCIB 10586 Q8L1B1 pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.5 native enzyme, and recombinant enzyme from Escherichia coli, amino acid sequence determination Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Pseudomonas fluorescens AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle tRNAIle from Pseudomonas fluorescens and Escherichia coli Pseudomonas fluorescens AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Pseudomonas fluorescens NCIB 10586 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle tRNAIle from Pseudomonas fluorescens and Escherichia coli Pseudomonas fluorescens NCIB 10586 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.5 ? x * 117000-117738, native isozyme IleRS-R2, SDS-PAGE and amino acid sequence determination Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.5 IleRS
-
 Pseudomonas fluorescens
6.1.1.5 IRS
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucine translase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucine--tRNA ligase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucine-transfer RNA ligase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucine-tRNA synthetase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucyl-transfer ribonucleate synthetase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucyl-transfer RNA synthetase
-
 Pseudomonas fluorescens
6.1.1.5 Isoleucyl-tRNA synthetase
-
 Pseudomonas fluorescens
6.1.1.5 Mupirocin resistance protein
-
 Pseudomonas fluorescens
6.1.1.5 Synthetase, isoleucyl-transfer ribonucleate
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.5 0.18
-
 L-isoleucine pH 7.5, recombinant isozyme IleRS-R1 Pseudomonas fluorescens
6.1.1.5 2.64
-
 L-isoleucine pH 7.5, recombinant isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 3 6 L-isoleucine pH 7.5, recombinant isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 3.3
-
 L-isoleucine pH 7.5, native isozyme IleRS-R2 Pseudomonas fluorescens
6.1.1.5 4.63
-
 L-isoleucine pH 7.5, native isozyme IleRS-R2 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.5 7.5
-
 assay at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.5 ATP
-
 Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.5 0.0000105
-
 pseudomonic acid pH 7.5, recombinant isozyme IleRS-R1, versus L-isoleucine Pseudomonas fluorescens
6.1.1.5 0.000015
-
 pseudomonic acid pH 7.5, recombinant isozyme IleRS-R1, versus ATP Pseudomonas fluorescens