Literature summary extracted from

Barends, T.R.; Polderman-Tijmes, J.J.; Jekel, P.A.; Hensgens, C.M.; de Vries, E.J.; Janssen, D.B.; Dijkstra, B.W.
The sequence and crystal structure of the alpha-amino acid ester hydrolase from Xanthomonas citri define a new family of beta-lactam antibiotic acylases (2003), J. Biol. Chem., 278, 23076-23084.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.43	expression in Escherichia coli	Xanthomonas citri

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.1.1.43	0.0015 ml of concentrated AEH is mixed with an equal volume of 12-15% polyethylene glycol 8000 in 100 mM cacodylate, pH 6.5, and equilibrated against 0.5 ml of this precipitant solution, crystals diffract to 1.9A	Xanthomonas citri

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.1.43	70915	-	4 * 70915, deduced from nucleotide sequence	Xanthomonas citri
3.1.1.43	72000	-	4 * 72000	Xanthomonas citri

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.1.1.43	an alpha-aminoacid ester + H2O	Xanthomonas citri	-	an alpha-amino acid + an alcohol	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.43	Xanthomonas citri	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.43	recombinant AEH	Xanthomonas citri

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.43	an alpha-aminoacid ester + H2O	-	Xanthomonas citri	an alpha-amino acid + an alcohol	-	?
3.1.1.43	an alpha-aminoacid ester + H2O	-	Xanthomonas citri	an alpha-amino acid + an alcohol	-	r

Subunits

EC Number	Subunits	Comment	Organism
3.1.1.43	tetramer	4 * 70915, deduced from nucleotide sequence	Xanthomonas citri
3.1.1.43	tetramer	4 * 72000	Xanthomonas citri

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.43	AEH	-	Xanthomonas citri

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Release 2023.1 (January 2023)