EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.5 | expression in Escherichia coli BL21(DE3)pLysE | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.3.4.5 | structures of enzyme complexed with intact ATP and substrates citrulline and aspartate, complexed with AMP and argininosuccinate, and complexed with AMP-PNP, arginine, aspartate and Mg2+, vapor diffusion method, X-ray analysis | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.5 | Mg2+ | required for maximal activity, in octahedral coordination with 3 oxygen atoms of the triphosphate group and 3 water molecules | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.5 | 44815 | - |
4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.5 | ATP + L-citrulline + L-aspartate | Thermus thermophilus | seventh step of the arginine biosynthesis, second step of the urea cycle | AMP + diphosphate + L-argininosuccinate | - |
? | |
6.3.4.5 | ATP + L-citrulline + L-aspartate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | seventh step of the arginine biosynthesis, second step of the urea cycle | AMP + diphosphate + L-argininosuccinate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.5 | Thermus thermophilus | P59846 | - |
- |
6.3.4.5 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P59846 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.5 | - |
Thermus thermophilus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.4.5 | ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate | detailed catalytic mechanism, stereochemistry | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.4.5 | 0.06 | - |
20°C | Thermus thermophilus |
6.3.4.5 | 0.92 | - |
70°C | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.5 | ATP + L-citrulline + L-aspartate | ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure | Thermus thermophilus | AMP + diphosphate + L-argininosuccinate | - |
r | |
6.3.4.5 | ATP + L-citrulline + L-aspartate | seventh step of the arginine biosynthesis, second step of the urea cycle | Thermus thermophilus | AMP + diphosphate + L-argininosuccinate | - |
? | |
6.3.4.5 | ATP + L-citrulline + L-aspartate | ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-argininosuccinate | - |
r | |
6.3.4.5 | ATP + L-citrulline + L-aspartate | seventh step of the arginine biosynthesis, second step of the urea cycle | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-argininosuccinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.5 | tetramer | 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.5 | Argininosuccinate synthetase | - |
Thermus thermophilus |
6.3.4.5 | ASS | - |
Thermus thermophilus |
6.3.4.5 | tAsS | - |
Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.4.5 | 8.5 | - |
assay at | Thermus thermophilus |