BRENDA - Enzyme Database show

Human microsomal prostaglandin E synthase-1: purification, functional characterization, and projection structure determination

Thoren, S.; Weinander, R.; Saha, S.; Jegerschoeld, C.; Pettersson, P.L.; Samuelsson, B.; Hebert, H.; Hamberg, M.; Morgenstern, R.; Jakobsson, P.J.; J. Biol. Chem. 278, 22199-22209 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression of histidine-tagged mPGES-1 in Escherichia coli
Homo sapiens
General Stability
EC Number
General Stability
Organism
5.3.99.3
activity in solubilized membranes remains stable for a prolonged time in the presence of reduced glutathione and glycerol
Homo sapiens
5.3.99.3
resistant to freeze-thawing
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.16
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
0.16
-
prostaglandin G2
-
Homo sapiens
5.3.99.3
0.71
-
glutathione
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17500
-
x * 17500, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
recombinant His-tagged mPGES-1, hydroxyapatite, Ni2+-affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
0.04
-
substrate 15-hydroperoxy-prostaglandin E2
Homo sapiens
5.3.99.3
0.043
-
substrate 5-hydroperoxyeicostetraenoic
Homo sapiens
5.3.99.3
0.17
-
substrate cumene hydroperoxide
Homo sapiens
5.3.99.3
0.81
-
substrate 1-chloro-2,4-dinitrobenzene
Homo sapiens
5.3.99.3
120
-
substrate (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
5.3.99.3
190
-
substrate prostaglandin G2
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
5.3.99.3
-20░C, 6 months, no loss of activity
Homo sapiens
5.3.99.3
4░C, solubilized membranes, 10 d, 50% loss of activity
Homo sapiens
5.3.99.3
4░C, solubilized membranes, 24 h, 30% loss of activity
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate + GSH
i.e. prostaglandin H2
652399
Homo sapiens
(5Z,13E)-(15S)-11a,15-dihydroxyprosta-5,13-dienoate + GSSG
i.e. prostaglandin E2
652399
Homo sapiens
?
5.3.99.3
1-chloro-2,4-dinitrobenzene + GSH
glutathione S-transferase activity
652399
Homo sapiens
?
-
652399
Homo sapiens
?
5.3.99.3
15-hydroperoxy-prostaglandin E2 + GSH
-
652399
Homo sapiens
prostaglandin E2 + GSSG
-
652399
Homo sapiens
?
5.3.99.3
5-hydroperoxyeicosatetraenoic acid + 2 GSH
-
652399
Homo sapiens
5-hydroxyeicosatetraenoic acid + GSSG + H2O
-
652399
Homo sapiens
?
5.3.99.3
cumene hydroperoxide + 2 GSH
-
652399
Homo sapiens
cumene hydroxide + GSSG + H2O
-
652399
Homo sapiens
?
5.3.99.3
prostaglandin G2 + GSH
-
652399
Homo sapiens
15-hydroperoxy-prostaglandine E2 + GSSG
-
652399
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17500, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
21
-
glutathione
-
Homo sapiens
5.3.99.3
50
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
75
-
prostaglandine G2
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression of histidine-tagged mPGES-1 in Escherichia coli
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.99.3
activity in solubilized membranes remains stable for a prolonged time in the presence of reduced glutathione and glycerol
Homo sapiens
5.3.99.3
resistant to freeze-thawing
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.16
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
0.16
-
prostaglandin G2
-
Homo sapiens
5.3.99.3
0.71
-
glutathione
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17500
-
x * 17500, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
recombinant His-tagged mPGES-1, hydroxyapatite, Ni2+-affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
0.04
-
substrate 15-hydroperoxy-prostaglandin E2
Homo sapiens
5.3.99.3
0.043
-
substrate 5-hydroperoxyeicostetraenoic
Homo sapiens
5.3.99.3
0.17
-
substrate cumene hydroperoxide
Homo sapiens
5.3.99.3
0.81
-
substrate 1-chloro-2,4-dinitrobenzene
Homo sapiens
5.3.99.3
120
-
substrate (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
5.3.99.3
190
-
substrate prostaglandin G2
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.99.3
-20░C, 6 months, no loss of activity
Homo sapiens
5.3.99.3
4░C, solubilized membranes, 10 d, 50% loss of activity
Homo sapiens
5.3.99.3
4░C, solubilized membranes, 24 h, 30% loss of activity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate + GSH
i.e. prostaglandin H2
652399
Homo sapiens
(5Z,13E)-(15S)-11a,15-dihydroxyprosta-5,13-dienoate + GSSG
i.e. prostaglandin E2
652399
Homo sapiens
?
5.3.99.3
1-chloro-2,4-dinitrobenzene + GSH
glutathione S-transferase activity
652399
Homo sapiens
?
-
652399
Homo sapiens
?
5.3.99.3
15-hydroperoxy-prostaglandin E2 + GSH
-
652399
Homo sapiens
prostaglandin E2 + GSSG
-
652399
Homo sapiens
?
5.3.99.3
5-hydroperoxyeicosatetraenoic acid + 2 GSH
-
652399
Homo sapiens
5-hydroxyeicosatetraenoic acid + GSSG + H2O
-
652399
Homo sapiens
?
5.3.99.3
cumene hydroperoxide + 2 GSH
-
652399
Homo sapiens
cumene hydroxide + GSSG + H2O
-
652399
Homo sapiens
?
5.3.99.3
prostaglandin G2 + GSH
-
652399
Homo sapiens
15-hydroperoxy-prostaglandine E2 + GSSG
-
652399
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17500, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
21
-
glutathione
-
Homo sapiens
5.3.99.3
50
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
75
-
prostaglandine G2
-
Homo sapiens