BRENDA - Enzyme Database

The structure of 3-methylaspartase from Clostridium tetanomorphum functions via the common enolase chemical step

Asuncion, M.; Blankenfeldt, W.; Barlow, J.N.; Gani, D.; Naismith, J.H.; J. Biol. Chem. 277, 8306-8311 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.1.2
sitting-drop vapur diffusion against a well solution of 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl pH 7.0 as precipitating solution and 16-22% ethylene glycol as additive, crystals diffract to 1.9 A
Clostridium tetanomorphum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
45500
-
2 * 45500
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Clostridium tetanomorphum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
recombinant 3-methylaspartase
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
-
652354
Clostridium tetanomorphum
mesaconate + NH3
-
652354
Clostridium tetanomorphum
r
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 45500
Clostridium tetanomorphum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.1.2
sitting-drop vapur diffusion against a well solution of 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl pH 7.0 as precipitating solution and 16-22% ethylene glycol as additive, crystals diffract to 1.9 A
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
45500
-
2 * 45500
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
recombinant 3-methylaspartase
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
-
652354
Clostridium tetanomorphum
mesaconate + NH3
-
652354
Clostridium tetanomorphum
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 45500
Clostridium tetanomorphum