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Literature summary extracted from

  • Asuncion, M.; Blankenfeldt, W.; Barlow, J.N.; Gani, D.; Naismith, J.H.
    The structure of 3-methylaspartase from Clostridium tetanomorphum functions via the common enolase chemical step (2002), J. Biol. Chem., 277, 8306-8311.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.2 expression in Escherichia coli Clostridium tetanomorphum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.2 sitting-drop vapur diffusion against a well solution of 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl pH 7.0 as precipitating solution and 16-22% ethylene glycol as additive, crystals diffract to 1.9 A Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 45500
-
 2 * 45500 Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum
-
 mesaconate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Clostridium tetanomorphum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.2 recombinant 3-methylaspartase Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate
-
 Clostridium tetanomorphum mesaconate + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer 2 * 45500 Clostridium tetanomorphum

Synonyms

EC Number Synonyms Comment Organism
4.3.1.2 MAL
-
 Clostridium tetanomorphum