EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.2.1 | - |
Rhodococcus erythropolis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.1 | 2,2'-dipyridyl | 2 mM, 30% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | 5,5'-dithio-bis-(2-nitrobenzoic acid) | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | 8-hydroxyquinoline | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | alloxan | competitive | Rhodococcus erythropolis | |
3.5.2.1 | Cd2+ | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | Co2+ | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | Cu2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
3.5.2.1 | cyanuric acid | competitive | Rhodococcus erythropolis | |
3.5.2.1 | dihydro-L-orotate | competitive | Rhodococcus erythropolis | |
3.5.2.1 | diisopropyl phosphofluoridate | 2 mM, 90% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | EDTA | 2 mM, 30% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | Hg2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
3.5.2.1 | N-bromosuccinimide | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | N-ethylmaleimide | 2 mM, complete inhibition | Rhodococcus erythropolis | |
3.5.2.1 | Ni2+ | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | o-phenanthroline | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
3.5.2.1 | p-chloromercuribenzoate | 2 mM, complete inhibition | Rhodococcus erythropolis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.1 | 1 | - |
Barbiturate | pH 8.0, 30°C | Rhodococcus erythropolis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.1 | Zn2+ | 4.4 mol per mol of enzyme | Rhodococcus erythropolis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.1 | 45000 | - |
4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
3.5.2.1 | 172000 | - |
gel filtration | Rhodococcus erythropolis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.95 | Rhodococcus erythropolis | - |
JCM 3132 | - |
3.5.1.95 | Rhodococcus erythropolis JCM 3132 | - |
JCM 3132 | - |
3.5.2.1 | Rhodococcus erythropolis | Q8RSQ2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.1 | - |
Rhodococcus erythropolis |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.2.1 | 4°C, 10% (v/v) ethylene glykol, stable up to 3 months | Rhodococcus erythropolis |
3.5.2.1 | 4°C, cell-free extract, loss of 70% of activity during 2 weeks | Rhodococcus erythropolis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.1 | barbiturate + H2O | strict specificity | Rhodococcus erythropolis | ureidomalonic acid | - |
? | |
3.5.2.1 | ureidomalonic acid | - |
Rhodococcus erythropolis | barbiturate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.2.1 | homotetramer | 4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
3.5.2.1 | More | partial amino acid sequence analysis | Rhodococcus erythropolis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.1 | 55 | - |
30 min, complete loss of activity | Rhodococcus erythropolis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.2.1 | 8 | - |
loss of activity above | Rhodococcus erythropolis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.1 | 0.42 | - |
cyanuric acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
3.5.2.1 | 1.7 | - |
alloxan | pH 8.0, 30°C | Rhodococcus erythropolis | |
3.5.2.1 | 4.5 | - |
dihydro-L-orotate | pH 8.0, 30°C | Rhodococcus erythropolis |