Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eroglu, B.; Powers-Lee, S.G.
    Unmasking a functional allosteric domain in an allosterically nonresponsive carbamoyl-phosphate synthetase (2002), J. Biol. Chem., 277, 45466-45472.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 IMP allosteric effect on Escherichia coli enzyme in dependence of temperature, no response to chimeric enzyme Escherichia coli
6.3.5.5 IMP the nonresponsive enzyme can bind UMP at domain D and allosteric effect on chimeric enzyme Saccharomyces cerevisiae
6.3.5.5 L-ornithine allosteric effect on chimeric enzyme and Escherichia coli enzyme Escherichia coli
6.3.5.5 L-ornithine allosteric effect on chimeric enzyme, no effect on Saccharomyces cerevisae enzyme Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.16
-
Saccharomyces cerevisiae
6.3.5.5 Escherichia coli and chimeric enzyme are expressed in Escherichia coli L673 Escherichia coli
6.3.5.5 expression in Saccharomyces pombe Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.16 D265A no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 D265E about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 D265N about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 D810A no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 D810E no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 D810N no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 H262N no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 H807N about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 I316G about 10% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 I316H no carbamoyl phosphate synthesis Saccharomyces cerevisiae
6.3.4.16 I316S about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 L229G about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae
6.3.4.16 S228A about 30% of the specific acitivity of the wild-type enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP effect on chimeric enzyme and Escherichia coli enzyme Escherichia coli
6.3.5.5 UMP the nonresponsive enzyme can bind UMP at domain D and allosteric effect on chimeric enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 additional information
-
additional information kinetic parameters for utilization of ATP and ADP by mutant enzymes Saccharomyces cerevisiae
6.3.5.5 0.361
-
L-glutamine chimeric enzyme, in presence of ornithine and absence of UMP Escherichia coli
6.3.5.5 0.89
-
L-glutamine Escherichia coli enzyme, in presence of ornithine and absence of UMP Escherichia coli
6.3.5.5 0.91
-
L-glutamine Escherichia coli enzyme, in absence of ornithine and UMP Escherichia coli
6.3.5.5 1.075
-
L-glutamine chimeric enzyme, in absence of ornithine and UMP Escherichia coli
6.3.5.5 1.49
-
L-glutamine Escherichia coli enzyme, in absence of ornithine and presence of UMP Escherichia coli
6.3.5.5 1.86
-
L-glutamine chimeric enzyme, in absence of ornithine and presence of UMP Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- Escherichia coli the product carbamoyl phosphate is utilized for the arginine biosynthesis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3- Saccharomyces cerevisiae the product carbamoyl phosphate is utilized for the arginine biosynthesis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3- Saccharomyces cerevisiae LPL26 the product carbamoyl phosphate is utilized for the arginine biosynthesis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Saccharomyces cerevisiae
-
LPL26
-
6.3.4.16 Saccharomyces cerevisiae LPL26
-
LPL26
-
6.3.5.5 Escherichia coli
-
and construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisiae enzyme
-
6.3.5.5 Saccharomyces cerevisiae
-
construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.5.5 side-chain modification construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme Escherichia coli
6.3.5.5 side-chain modification construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5
-
Saccharomyces cerevisiae
6.3.5.5 Escherichia coli and chimeric enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + L-Gln + HCO3-
-
Saccharomyces cerevisiae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.4.16 2 ATP + L-Gln + HCO3-
-
Saccharomyces cerevisiae LPL26 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.4.16 2 ATP + NH4+ + HCO3-
-
Saccharomyces cerevisiae 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.4.16 2 ATP + NH4+ + HCO3-
-
Saccharomyces cerevisiae LPL26 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Saccharomyces cerevisiae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is utilized for the arginine biosynthesis Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is utilized for the arginine biosynthesis Saccharomyces cerevisiae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Saccharomyces cerevisiae LPL26 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is utilized for the arginine biosynthesis Saccharomyces cerevisiae LPL26 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
Saccharomyces cerevisiae 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
Saccharomyces cerevisiae LPL26 2 ADP + phosphate + carbamoyl phosphate
-
?