EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.5 | IMP | allosteric effect on Escherichia coli enzyme in dependence of temperature, no response to chimeric enzyme | Escherichia coli | |
6.3.5.5 | IMP | the nonresponsive enzyme can bind UMP at domain D and allosteric effect on chimeric enzyme | Saccharomyces cerevisiae | |
6.3.5.5 | L-ornithine | allosteric effect on chimeric enzyme and Escherichia coli enzyme | Escherichia coli | |
6.3.5.5 | L-ornithine | allosteric effect on chimeric enzyme, no effect on Saccharomyces cerevisae enzyme | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.16 | - |
Saccharomyces cerevisiae |
6.3.5.5 | Escherichia coli and chimeric enzyme are expressed in Escherichia coli L673 | Escherichia coli |
6.3.5.5 | expression in Saccharomyces pombe | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.16 | D265A | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | D265E | about 10% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | D265N | about 10% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | D810A | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | D810E | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | D810N | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | H262N | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | H807N | about 30% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | I316G | about 10% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | I316H | no carbamoyl phosphate synthesis | Saccharomyces cerevisiae |
6.3.4.16 | I316S | about 30% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | L229G | about 30% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
6.3.4.16 | S228A | about 30% of the specific acitivity of the wild-type enzyme | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.5 | UMP | effect on chimeric enzyme and Escherichia coli enzyme | Escherichia coli | |
6.3.5.5 | UMP | the nonresponsive enzyme can bind UMP at domain D and allosteric effect on chimeric enzyme | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.16 | additional information | - |
additional information | kinetic parameters for utilization of ATP and ADP by mutant enzymes | Saccharomyces cerevisiae | |
6.3.5.5 | 0.361 | - |
L-glutamine | chimeric enzyme, in presence of ornithine and absence of UMP | Escherichia coli | |
6.3.5.5 | 0.89 | - |
L-glutamine | Escherichia coli enzyme, in presence of ornithine and absence of UMP | Escherichia coli | |
6.3.5.5 | 0.91 | - |
L-glutamine | Escherichia coli enzyme, in absence of ornithine and UMP | Escherichia coli | |
6.3.5.5 | 1.075 | - |
L-glutamine | chimeric enzyme, in absence of ornithine and UMP | Escherichia coli | |
6.3.5.5 | 1.49 | - |
L-glutamine | Escherichia coli enzyme, in absence of ornithine and presence of UMP | Escherichia coli | |
6.3.5.5 | 1.86 | - |
L-glutamine | chimeric enzyme, in absence of ornithine and presence of UMP | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.5.5 | 2 ATP + L-Gln + HCO3- | Escherichia coli | the product carbamoyl phosphate is utilized for the arginine biosynthesis | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | Saccharomyces cerevisiae | the product carbamoyl phosphate is utilized for the arginine biosynthesis | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | Saccharomyces cerevisiae LPL26 | the product carbamoyl phosphate is utilized for the arginine biosynthesis | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.16 | Saccharomyces cerevisiae | - |
LPL26 | - |
6.3.4.16 | Saccharomyces cerevisiae LPL26 | - |
LPL26 | - |
6.3.5.5 | Escherichia coli | - |
and construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisiae enzyme | - |
6.3.5.5 | Saccharomyces cerevisiae | - |
construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
6.3.5.5 | side-chain modification | construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme | Escherichia coli |
6.3.5.5 | side-chain modification | construction of a chimeric enzyme, in which the C-terminal 136 residues of Escherichia coli enzyme are replaced by the corresponding residues of Saccharomyces cerevisae enzyme | Saccharomyces cerevisiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.5.5 | - |
Saccharomyces cerevisiae |
6.3.5.5 | Escherichia coli and chimeric enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.16 | 2 ATP + L-Gln + HCO3- | - |
Saccharomyces cerevisiae | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + L-Gln + HCO3- | - |
Saccharomyces cerevisiae LPL26 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Saccharomyces cerevisiae | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Saccharomyces cerevisiae LPL26 | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Saccharomyces cerevisiae | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | the product carbamoyl phosphate is utilized for the arginine biosynthesis | Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | the product carbamoyl phosphate is utilized for the arginine biosynthesis | Saccharomyces cerevisiae | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Saccharomyces cerevisiae LPL26 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | the product carbamoyl phosphate is utilized for the arginine biosynthesis | Saccharomyces cerevisiae LPL26 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + NH4+ + HCO3- | - |
Saccharomyces cerevisiae | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + NH4+ + HCO3- | - |
Saccharomyces cerevisiae LPL26 | 2 ADP + phosphate + carbamoyl phosphate | - |
? |