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Literature summary extracted from

  • Roje, S.; Janave, M.T.; Ziemak, M.J.; Hanson, A.D.
    Cloning and characterization of mitochondrial 5-formyltetrahydrofolate cycloligase from higher plants (2002), J. Biol. Chem., 277, 42748-42754.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 DNA sequence determination and analysis, expression in Escherichia coli BL21 as full length or truncated protein, the latter lacking the mitochondrial targeting sequence, functional complementation of enzyme deficient yeast fau1 mutant, yeast strain WHY1.3.1 Arabidopsis thaliana
6.3.3.2 DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as full length or truncated protein, the latter lacking the mitochondrial targeting sequence Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.3.2 mitochondrion exclusively Pisum sativum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.2 26500
-
 1 * 26500, recombinant truncated enzyme, amino acid sequence determination Arabidopsis thaliana
6.3.3.2 27000
-
 recombinant truncated enzyme, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate Pisum sativum
-
 ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate Arabidopsis thaliana
-
 ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ir
6.3.3.2 ATP + 5-formyltetrahydrofolate Solanum lycopersicum
-
 ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Arabidopsis thaliana Q8L539
-
-
6.3.3.2 Pisum sativum
-
-
-
6.3.3.2 Solanum lycopersicum Q8LKF6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2 to near homogeneity, recombinant enzyme from Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.3.2 leaf high content Pisum sativum
-
6.3.3.2 root low content Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 additional information
-
 activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme Arabidopsis thaliana
6.3.3.2 additional information
-
 activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Arabidopsis thaliana ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ir
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Pisum sativum ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Arabidopsis thaliana ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ir
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Solanum lycopersicum ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ir
6.3.3.2 ATP + 5-formyltetrahydrofolate-pentaglutamate preferred substrate to the monoglutamyl form Arabidopsis thaliana ADP + phosphate + 5,10-methylenetetrahydrofolate-pentaglutamate
-
 ir
6.3.3.2 additional information enzyme is specific for the 6S-isomer Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 monomer 1 * 26500, recombinant truncated enzyme, amino acid sequence determination Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.3.3.2 5-FCL
-
 Pisum sativum
6.3.3.2 5-FCL
-
 Arabidopsis thaliana
6.3.3.2 5-FCL
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 5.3
-
 5-formyltetrahydrofolate truncated recombinant enzyme, pH 6.0, 23°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.3.2 8.5
-
 bicine buffer Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.3.2 5.5 9 linear increase of activity to maximum at pH 8.5 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.2 ATP dependent on Pisum sativum
6.3.3.2 ATP dependent on Arabidopsis thaliana
6.3.3.2 ATP dependent on Solanum lycopersicum