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Literature summary extracted from

  • Oza, S.L.; Tetaud, E.; Ariyanayagam, M.R.; Warnon, S.S.; Fairlamb, A.H.
    A Single Enzyme Catalyses Formation of Trypanothione from Glutathione and Spermidine in Trypanosoma cruzi (2002), J. Biol. Chem., 277, 35853-35861.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.9 expression in Escherichia coli and Saccharomyces cerevisiae Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.9 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid above 100 mM Trypanosoma cruzi
6.3.1.9 ammonium sulfate almost complete inhibition at 500 mM Trypanosoma cruzi
6.3.1.9 KCl approx. 50% inhibition at 500 mM Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.9 0.053
-
 ATP pH 8.0 Trypanosoma cruzi
6.3.1.9 0.066
-
 N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine pH 8.0 Trypanosoma cruzi
6.3.1.9 0.19
-
 GSH pH 8.0, with N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 Mg2+ required for activity Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.9 64000
-
 gel filtration Trypanosoma cruzi
6.3.1.9 73560
-
 deduced from nucleotide sequence Trypanosoma cruzi
6.3.1.9 73740
-
 MALDI mass spectrometry Trypanosoma cruzi
6.3.1.9 73744
-
 1 * 73744, MALDI mass spectrometry Trypanosoma cruzi
6.3.1.9 74500
-
 SDS-PAGE Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.9 gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP Trypanosoma cruzi
-
 N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate trivial name trypanothione ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.9 Trypanosoma cruzi Q9GT49
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9 recombinant His-tagged TcTryS, Ni2+-affinity, anion exchange Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.9 0.77
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
-
 Trypanosoma cruzi N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate trivial name trypanothione ?
6.3.1.9 gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP multifunctional enzyme having also glutathionylspermidine synthetase, glutathionylaminopropylcadaverine synthetase, homotrypanothione synthetase and a weak amidase activity Trypanosoma cruzi N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate trivial name trypanothione ?

Subunits

EC Number Subunits Comment Organism
6.3.1.9 monomer 1 * 73560, deduced from nucleotide sequence Trypanosoma cruzi
6.3.1.9 monomer 1 * 73744, MALDI mass spectrometry Trypanosoma cruzi
6.3.1.9 monomer 1 * 74500, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 TcTryS
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.9 2.8
-
 N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine pH 8.0 Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 8.1
-
-
 Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.9 5.5 10 approx. 50% of maximal activity at pH 6.6 and pH 9.5 respectively Trypanosoma cruzi