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Literature summary extracted from

  • Samanani, N.; Facchini, P.J.
    Purification and characterization of norcoclaurine synthase. The first committed enzyme in benzylisoquinoline alkaloid biosynthesis in plants (2002), J. Biol. Chem., 277, 33878-33883.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.78 norlaudanosoline product inhibition, kinetics Thalictrum flavum ssp. glaucum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.78 additional information
-
 additional information sigmoidal saturation kinetics for dopamine, cooperativity between the dopamine binding sites on each subunit Thalictrum flavum ssp. glaucum
4.2.1.78 0.335
-
 4-hydroxyphenylacetaldehyde pH 7.5, 37°C, hyperbolic saturation kinetics Thalictrum flavum ssp. glaucum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.78 additional information the enzyme is not affected by divalent cations Thalictrum flavum ssp. glaucum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.78 15000
-
 2 * 15000, SDS-PAGE Thalictrum flavum ssp. glaucum
4.2.1.78 28000
-
 gel filtration Thalictrum flavum ssp. glaucum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde Thalictrum flavum ssp. glaucum first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux (S)-Norcoclaurine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.78 Thalictrum flavum ssp. glaucum
-
 i.e. meadow rue, 4 isoelectrically different isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.78 1590fold to homogeneity from cell suspension culture Thalictrum flavum ssp. glaucum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.78 4-hydroxyphenylacetaldehyde + dopamine = (S)-norcoclaurine + H2O the reaction makes a six-membered ring by forming a bond between C-6 of the 3,4-dihydroxyphenyl group of the dopamine and C-1 of the aldehyde in the imine formed between the substrates, the product is the precursor of the benzylisoquinoline alkaloids in plants, the enzyme, formerly known as (S)-norleudanosoline synthase, will also catalyse the reaction of 4-(2-aminoethyl)benzene-1,2-diol + (di-3,4-hydroxyphenyl)acetaldehyde to form (S)-norlaudanosoline, but this alkaloid has not been found to occur in plants, iso-ordered bi-uni mechanism with 4-hydroxyphenylacetaldehyde binding before dopamine Thalictrum flavum ssp. glaucum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.78 cell suspension culture
-
 Thalictrum flavum ssp. glaucum
-
4.2.1.78 flower bud low enzyme activity Thalictrum flavum ssp. glaucum
-
4.2.1.78 leaf
-
 Thalictrum flavum ssp. glaucum
-
4.2.1.78 root highest enzyme activity Thalictrum flavum ssp. glaucum
-
4.2.1.78 stem
-
 Thalictrum flavum ssp. glaucum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.78 0.09
-
 purified enzyme Thalictrum flavum ssp. glaucum

Storage Stability

EC Number Storage Stability Organism
4.2.1.78 -80°C, purified enzyme, 7 days, no loss of activity Thalictrum flavum ssp. glaucum
4.2.1.78 4°C, purified enzyme, 7 days, 75% loss of activity Thalictrum flavum ssp. glaucum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.78 4-(2-aminoethyl)benzene-1,2-diol + 3,4-dihydroxyphenylacetaldehyde
-
 Thalictrum flavum ssp. glaucum (S)-norlaudanosoline + H2O
-
 ?
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde 4-(2-aminoethyl)benzene-1,2-diol is dopmaine, cooperativity between the dopamine binding sites on each subunit Thalictrum flavum ssp. glaucum (S)-Norcoclaurine + H2O
-
 ?
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde first step of benzylisoquinoline alkaloid synthesis, enzyme might play a regulatory or rate-limiting role in controlling the rate of pathway flux Thalictrum flavum ssp. glaucum (S)-Norcoclaurine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.78 dimer 2 * 15000, SDS-PAGE Thalictrum flavum ssp. glaucum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.78 (S)-Norlaudanosoline synthase
-
 Thalictrum flavum ssp. glaucum
4.2.1.78 NCS
-
 Thalictrum flavum ssp. glaucum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.78 42 55
-
 Thalictrum flavum ssp. glaucum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.78 37 57 half-maximal activities at 37°C and 57°C Thalictrum flavum ssp. glaucum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.78 6.5 7
-
 Thalictrum flavum ssp. glaucum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.78 4.7
-
 norlaudanosoline pH 7.5, 37°, with respect to dopamine Thalictrum flavum ssp. glaucum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.78 Thalictrum flavum ssp. glaucum isoelectric focusing, minor isoform
-
 5.5
4.2.1.78 Thalictrum flavum ssp. glaucum isoelectric focusing, major isoform
-
 5.7
4.2.1.78 Thalictrum flavum ssp. glaucum isoelectric focusing, major isoform
-
 6
4.2.1.78 Thalictrum flavum ssp. glaucum isoelectric focusing, minor isoform
-
 6.2