Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rashid, N.; Imanaka, H.; Kanai, T.; Fukui, T.; Atomi, H.; Imanaka, T.
    A novel candidate for the true fructose-1,6-bisphosphatase in Archaea (2002), J. Biol. Chem., 277, 30649-30655.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 dithioerythritol in combination wit Mg2+ Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11
-
 Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 D-fructose-2,6-bisphosphate
-
 Thermococcus kodakarensis
3.1.3.11 additional information not: AMP Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.1
-
 D-fructose 1,6-bisphosphate 95°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+
-
 Thermococcus kodakarensis
3.1.3.11 Mn2+ 1 mM, 4fold increase of activity Thermococcus kodakarensis
3.1.3.11 additional information not: Ca2+, Ni2+ Thermococcus kodakarensis
3.1.3.11 Zn2+ 1 mM Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 42000
-
 8 * 42000, SDS-PAGE Thermococcus kodakarensis
3.1.3.11 340000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Thermococcus kodakarensis enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Thermococcus kodakarensis Q8NKR9 KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 the recombinant enzyme Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Thermococcus kodakarensis D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Thermococcus kodakarensis D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 additional information not: D-fructose 1-phosphate, D-fructose 6-phosphate, D-fructose 2,6-bisphosphate, glycerol 3-phosphate, glycerol 1-phosphate Thermococcus kodakarensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 octamer 8 * 42000, SDS-PAGE Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.11 95
-
 in presence of 20 mM Mg2+ and 20 mM dithioerythritol Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.11 37 95 the enzyme shows a nearly linear increase in activity between 37°C and 95°C Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.11 100
-
 half-life of 150 min in boiling water Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 17
-
 D-fructose 1,6-bisphosphate 95°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8
-
 in presence of 20 mM Mg2+ and 20 mM dithioerythritol Thermococcus kodakarensis