Literature summary extracted from
Lawrence, A.J.; Coote, J.G.; Kazi, Y.F.; Lawrence, P.D.; MacDonald-Fyall, J.; Orr, B.M.; Parton, R.; Riehle, M.; Sinclair, J.; Young, J.; Price, N.C.
A direct pyrophosphatase-coupled assay provides new insights into the activation of the secreted adenylate cyclase from Bordetella pertussis by calmodulin (2002), J. Biol. Chem., 277, 22289-22296.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.6.1.1 |
Calmodulin |
strong activation |
Bordetella pertussis |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.6.1.1 |
expression in Escherichia coli |
Bordetella pertussis |
General Stability
EC Number |
General Stability |
Organism |
---|
4.6.1.1 |
calmodulin stablizes CyaA |
Bordetella pertussis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.6.1.1 |
EGTA |
freshly activated CyaA is inhibited by EGTA at low calmodulin concentrations |
Bordetella pertussis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.6.1.1 |
ATP |
Bordetella pertussis |
- |
3',5'-cyclic AMP + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.1 |
Bordetella pertussis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.1 |
recombinant CyaA |
Bordetella pertussis |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.6.1.1 |
-20°C, 8 M urea, no loss of activity |
Bordetella pertussis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.6.1.1 |
ATP |
- |
Bordetella pertussis |
3',5'-cyclic AMP + diphosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.1 |
adenylate cyclase toxin |
- |
Bordetella pertussis |
4.6.1.1 |
CyaA |
- |
Bordetella pertussis |