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Literature summary extracted from

  • Verhees, C.H.; Huynen, M.A.; Ward, D.E.; Schiltz, E.; De Vos, W.M.; van der Oost, J.
    The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily (2001), J. Biol. Chem., 276, 40926-40932.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.9 expression in Escherichia coli BL21 Pyrococcus furiosus
5.3.1.9 functionally expressed in Escherichia coli BL21(DE3) Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.9 D-fructose 1,6-bisphosphate 10 mM, residual activities are 41% and 53% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
5.3.1.9 D-Fructose 1-phosphate 2 mM, residual activities are 50% and 69% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
5.3.1.9 D-mannose 6-phosphate 1.25 mM, residual activities are 18% and 38% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
5.3.1.9 additional information activity is not affected by addition of 10 mM EDTA. The addition of fructose, glucose, mannose, galactose (10 mM), pyruvate, phosphoenolpyruvate (10 mM), AMP, ADP, or ATP (3.5 mM), does not show any effect on the activity neither in the fructose 6-phosphate formation, nor in the glucose 6-phosphate formation Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.9 0.42
-
 D-fructose 6-phosphate 50°C, pH 7.0, recombinant enzyme Pyrococcus furiosus
5.3.1.9 0.6 3 D-fructose 6-phosphate pH 7.0, 50°C Pyrococcus furiosus
5.3.1.9 0.63
-
 D-fructose 6-phosphate 50°C, pH 7.0, native enzyme Pyrococcus furiosus
5.3.1.9 1.99
-
 D-glucose 6-phosphate pH 7.0, 50°C Pyrococcus furiosus
5.3.1.9 1.99
-
 D-glucose 6-phosphate 50°C, pH 7.0, native enzyme Pyrococcus furiosus
5.3.1.9 2
-
 D-glucose 6-phosphate 50°C, pH 7.0, recombinant enzyme Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.9 additional information activity is not affected by addition of Mg2+ or Mn2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.9 21476
-
 2 * 21476, calculated from sequence Pyrococcus furiosus
5.3.1.9 23000
-
 2 * 23000, SDS-PAGE Pyrococcus furiosus
5.3.1.9 23500
-
 2 * 23500, SDS-PAGE Pyrococcus furiosus
5.3.1.9 49600
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.9 Pyrococcus furiosus P83194
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.9
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.9 14.5
-
 50°C, pH 7.0, reaction with D-fructose 6-phosphate Pyrococcus furiosus
5.3.1.9 14.5
-
 pH 7.0, 50°C, substrate: D-fructose 6-phosphate Pyrococcus furiosus
5.3.1.9 29.1
-
 50°C, pH 7.0, reaction with D-glucose 6-phosphate Pyrococcus furiosus
5.3.1.9 29.1
-
 pH 7.0, 50°C, substrate: D-glucose 6-phosphate Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.9 D-fructose 6-phosphate
-
 Pyrococcus furiosus D-glucose 6-phosphate
-
 r
5.3.1.9 D-glucose 6-phosphate
-
 Pyrococcus furiosus D-fructose 6-phosphate
-
 r
5.3.1.9 additional information enzyme does not convert mannose 6-phosphate to fructose 6-phosphate Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.9 dimer 2 * 23000, SDS-PAGE Pyrococcus furiosus
5.3.1.9 homodimer 2 * 23500, SDS-PAGE Pyrococcus furiosus
5.3.1.9 homodimer 2 * 21476, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.9 pgiA
-
 Pyrococcus furiosus
5.3.1.9 Phosphoglucose isomerase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.9 50
-
 assay at Pyrococcus furiosus
5.3.1.9 90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.9 70 115 70°C: about 40% of maximal activity, 115°C: about 50% of maximal activity Pyrococcus furiosus
5.3.1.9 70 110 70°C: about 40% of maximal activity, 110°C: about 35% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.9 90
-
 half-life: 2.4 h Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.9 7
-
 both directions Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.9 6 8.5 the enzyme shows reversible isomerization activity with fructose 6-phosphate and glucose 6-phosphate between pH 6.0 and pH 8.5 Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
5.3.1.9 physiological function the enzyme is part of the Pyrococcus furiosus variant of the Embden-Meyerhof pathway Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.9 6.2
-
 D-glucose 6-phosphate pH 7.0, 50°C, native enzyme Pyrococcus furiosus
5.3.1.9 8.5
-
 D-glucose 6-phosphate pH 7.0, 50°C, recombinant enzyme Pyrococcus furiosus
5.3.1.9 11.5
-
 D-fructose 6-phosphate pH 7.0, 50°C, native enzyme Pyrococcus furiosus
5.3.1.9 16.5
-
 D-fructose 6-phosphate pH 7.0, 50°C, recombinant enzyme Pyrococcus furiosus