Literature summary extracted from

Sekine, S.; Shimada, A.; Nureki, O.; Cavarelli, J.; Moras, D.; Vassylyev, D.G.; Yokoyama, S.
Crucial role of the high-loop lysine for the catalytic activity of arginyl-tRNA synthetase (2001), J. Biol. Chem., 276, 3723-3726.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.1.1.19	expression of the wild type and K116G mutant in Escherichia coli	Thermus thermophilus

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.1.1.19	K116G	expression of the mutant in Escherichia coli	Thermus thermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.19	Thermus thermophilus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.1.1.19	purification of the wild type and K116G mutant, expressed in Escherichia coli, by DEAE-Sephacel and PhenylSuperose column chromatography	Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.1.1.19	additional information	-	K116G mutant displays very low activity compared to wild type enzyme	Thermus thermophilus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.1.1.19	ATP + L-arginine + tRNAArg	-	Thermus thermophilus	AMP + diphosphate + L-arginyl-tRNAArg	-	?

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Release 2023.1 (January 2023)