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Literature summary extracted from

  • Ramesh, V.; RajBhandary, U.L.
    Importance of the anticodon sequence in the aminoacylation of tRNAs by methionyl-tRNA synthetase and by valyl-tRNA synthetase in an archaebacterium (2001), J. Biol. Chem., 276, 3660-3665.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 subcloning in Escherichia coli strain XL1-blue, expression in Haloferax volcanii Haloferax volcanii

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.9 additional information construction of mutant tRNAs Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal Haloferax volcanii
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet Haloferax volcanii protein biosynthesis in archaea is initiated with methionine not with formylmethionine AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-methionine + tRNAMet Haloferax volcanii WFD11 protein biosynthesis in archaea is initiated with methionine not with formylmethionine AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 Haloferax volcanii CAU to GAC anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 Haloferax volcanii WFD11 CAU to GAC anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 additional information Haloferax volcanii no activity with the CAU to CUA initiator tRNAMet mutant in vivo ?
-
 ?
6.1.1.10 additional information Haloferax volcanii WFD11 no activity with the CAU to CUA initiator tRNAMet mutant in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Haloferax volcanii
-
-
-
6.1.1.9 Haloferax volcanii WFD11
-
-
-
6.1.1.10 Haloferax volcanii
-
-
-
6.1.1.10 Haloferax volcanii WFD11
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.10 ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet the anticodon sequence is important for tRNA substrate specificity Haloferax volcanii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAiMet,GAC mutant anticodon CAU to GAC initiator tRNA mutant, i.e. G34C36 mutant, is aminoacylated in vitro and in vivo, its anticodon sequence corresponds to the one of tRNAVal Haloferax volcanii AMP + diphosphate + L-valyl-tRNAiMet,GAC mutant
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Haloferax volcanii AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal tRNA recognition mechanism, the anticodon sequence is important for the aminoacylation Haloferax volcanii AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Haloferax volcanii AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-methionine + tRNAMet protein biosynthesis in archaea is initiated with methionine not with formylmethionine Haloferax volcanii AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Haloferax volcanii WFD11 AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-methionine + tRNAMet protein biosynthesis in archaea is initiated with methionine not with formylmethionine Haloferax volcanii WFD11 AMP + diphosphate + L-methionyl-tRNAMet
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction Haloferax volcanii AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 CAU to GAC anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction Haloferax volcanii AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction Haloferax volcanii WFD11 AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 ATP + L-valine + tRNAMetG34C36 CAU to GAC anticodon mutant initiator tRNAMet, reduced reverse, i.e. deacetylation, reaction Haloferax volcanii WFD11 AMP + diphosphate + L-valyl-tRNAMetG34C36
-
 r
6.1.1.10 additional information no activity with the CAU to CUA initiator tRNAMet mutant in vivo Haloferax volcanii ?
-
 ?
6.1.1.10 additional information no activity with the CAU to CUA initiator tRNAMet mutant in vivo Haloferax volcanii WFD11 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.9 G7a
-
 Haloferax volcanii
6.1.1.9 Synthetase, valyl-transfer ribonucleate
-
 Haloferax volcanii
6.1.1.9 Valine transfer ribonucleate ligase
-
 Haloferax volcanii
6.1.1.9 Valine translase
-
 Haloferax volcanii
6.1.1.9 Valine--tRNA ligase
-
 Haloferax volcanii
6.1.1.9 ValRS
-
 Haloferax volcanii
6.1.1.9 Valyl transfer ribonucleic acid synthetase
-
 Haloferax volcanii
6.1.1.9 Valyl-transfer ribonucleate synthetase
-
 Haloferax volcanii
6.1.1.9 Valyl-transfer RNA synthetase
-
 Haloferax volcanii
6.1.1.9 Valyl-tRNA ligase
-
 Haloferax volcanii
6.1.1.9 Valyl-tRNA synthetase
-
 Haloferax volcanii
6.1.1.10 Methionine translase
-
 Haloferax volcanii
6.1.1.10 Methionine--tRNA ligase
-
 Haloferax volcanii
6.1.1.10 Methionyl tRNA synthetase
-
 Haloferax volcanii
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Haloferax volcanii
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Haloferax volcanii
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Haloferax volcanii
6.1.1.10 MetRS
-
 Haloferax volcanii
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.9 ATP
-
 Haloferax volcanii
6.1.1.10 ATP
-
 Haloferax volcanii