Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishino, T.; Komori, K.; Ishino, Y.; Morikawa, K.
    Dissection of the regional roles of the archaeal Holliday junction resolvase Hjc by structural and mutational analyses (2001), J. Biol. Chem., 276, 35735-35740.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.10 expression of mutant enzymes in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.10 selenomethionine-containing enzyme crystallized by microbatch method with a silicone oil overlay, hexagonal form of the enzyme Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.10 D33A mutant enzyme retains proper binding ablity to the Holliday junction Pyrococcus furiosus
3.1.21.10 DELTA1-5 mutation causes a considerable decrease in Hjc-Holliday junction complex formation and cleavage activity Pyrococcus furiosus
3.1.21.10 K30A/K31A mutant enzyme retains proper binding ability to the Holliday junction, little or almost no cleavage activity Pyrococcus furiosus
3.1.21.10 K51A/K52A mutant enzyme retains proper binding ability to the Holliday junction, weak cleavage activity Pyrococcus furiosus
3.1.21.10 R3A/K4A mutation reduces the activity by 20fold as compared with the wild-type enzyme. The binding to the Holliday junction is substantially lowered Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.10 DNA + H2O Holliday junction cleavage using 4Jh and Z28 Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.10 holliday junction resolvase hjc
-
 Pyrococcus furiosus