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Literature summary extracted from

  • Reddy, S.K.; Kamireddi, M.; Dhanireddy, K.; Young, L.; Davis, A.; Reddy, P.T.
    Eukaryotic-like adenylyl cyclases in Mycobacterium tuberculosis H37Rv: cloning and characterization (2001), J. Biol. Chem., 276, 35141-35149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 R18A 88% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R18A/R19G 67% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R19G 78% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R27A 78% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R31G 118% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R43A 61% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R43A/R44G 1.3% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R43K 77% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R43K/R44K 39% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R44G 41% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R44K 116% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R46A 85% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R46K 135% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis
4.6.1.1 R4G 77% of wild-type activity, in the presence of 2 mM Mn2+ Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.1 0.05
-
 ATP recombinant adenylyl cclase, in the presence of Mn2+ Mycobacterium tuberculosis
4.6.1.1 3.5
-
 ATP recombinant adenylyl cclase, in the presence of Mg2+ Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane six putative helical transmembrane domains Mycobacterium tuberculosis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mn2+ 1 mM, 47fold activation Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.1 45000
-
 x * 45000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Mycobacterium tuberculosis
-
 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP Mycobacterium tuberculosis H37Rv
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Mycobacterium tuberculosis P9WQ35
-
-
4.6.1.1 Mycobacterium tuberculosis H37Rv P9WQ35
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 recombinant adenylyl cyclase Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.1 0.48
-
 in the presence of physiological ATP concentrations and 1 mM Mn2+ Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Mycobacterium tuberculosis 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Mycobacterium tuberculosis H37Rv 3',5'-cyclic AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.1 ? x * 45000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Mycobacterium tuberculosis
4.6.1.1 Rv1625c
-
 Mycobacterium tuberculosis