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Literature summary extracted from

  • Hauser, F.; Strassner, J.; Schaller, A.
    Cloning, expression, and characterization of tomato (Lycopersicon esculentum) aminopeptidase P (2001), J. Biol. Chem., 276, 31732-31737.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9 gene LeAPP1, DNA and amino acid sequence determination and analysis Solanum lycopersicum
3.4.11.9 gene LeAPP2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli in N-terminal fusion with GST, a mutant form lacking 11 amino acids from the N-terminus is not active Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 1,10-phenanthroline 50% inhibition at 0.036 mM Solanum lycopersicum
3.4.11.9 2-mercaptoethanol 50% inhibition at 0.043 mM Solanum lycopersicum
3.4.11.9 Ca2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
3.4.11.9 Co2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
3.4.11.9 Cu2+ complete inhibition at 4 mM Solanum lycopersicum
3.4.11.9 diethyldicarbonate 50% inhibition at 0.011 mM Solanum lycopersicum
3.4.11.9 EDTA 50% inhibition at 0.34 m Solanum lycopersicum
3.4.11.9 NEM 50% inhibition at 0.079 mM Solanum lycopersicum
3.4.11.9 Zn2+ complete inhibition at 4 mM Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.9 cytosol
-
Solanum lycopersicum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Ca2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
3.4.11.9 Co2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
3.4.11.9 Mg2+ stimulation of the enzyme Solanum lycopersicum
3.4.11.9 Mn2+ stimulation of the enzyme, most effective at 4 mM Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 105000
-
x * 105000, recombinant GST-fusion protein, SDS-PAGE Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Solanum lycopersicum Q93X45 gene LeAPP2, isozyme APP2
-
3.4.11.9 Solanum lycopersicum Q93X46 gene LeAPP1, isozyme APP1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9 recombinant isozyme APP2 from Escherichia coli Solanum lycopersicum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.9 release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide isozyme APP-2 shows a preference for Arg-Pro-Pro-, Arg-Pro-Lys-, Pro-Pro-Gly-, -Phe-Gly- in descending order Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.9 additional information
-
-
Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
3.4.11.9 -20°C to 4°C, 0.5 mg/ml recombinant GST-fusion enzyme, several weeks without significant loss of activity Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 bradykinin + H2O i.e. RPPGFSPFR, rapid hydrolysis of the N-terminal Arg1-Pro2 bond Solanum lycopersicum Arg + PPGFSPFR
-
?
3.4.11.9 additional information systemin, a peptide hormone-like signaling molecule from tomato plants, is no substrate Solanum lycopersicum ?
-
?
3.4.11.9 Nalpha-(2-aminobenzoyl)-Lys-Pro-Pro 4-nitroanilide + H2O
-
Solanum lycopersicum Nalpha-(2-aminobenzoyl)-Lys + Pro-Pro-4-nitroanilide
-
?
3.4.11.9 PPGFSPFR + H2O low activity Solanum lycopersicum Pro + PGFSPFR
-
?
3.4.11.9 substance P + H2O i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond Solanum lycopersicum Arg + PKPQQFFGLM
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 ? x * 105000, recombinant GST-fusion protein, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.9 aminopeptidase P
-
Solanum lycopersicum
3.4.11.9 LeAPP1
-
Solanum lycopersicum
3.4.11.9 LeAPP2
-
Solanum lycopersicum
3.4.11.9 More the enzyme belongs to the peptidase family M24 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 7.5
-
assay at Solanum lycopersicum