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Literature summary extracted from

  • Lee, J.; Gravel, M.; Gao, E.; O'Neill, R.C.; Braun, P.E.
    Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity (2001), J. Biol. Chem., 276, 14804-14813.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.37 wild-type and mutant CNP1, expression in Escherichia coli BL21 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.37 C231A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C231S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C236A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C236S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C314A CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C314S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 C397S CNP1 catalytic fragment mutant, kinetics Rattus norvegicus
3.1.4.37 H230F almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
3.1.4.37 H230L almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
3.1.4.37 H309F almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
3.1.4.37 H309L almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 Rattus norvegicus
3.1.4.37 additional information CNP1: N-terminal deletion mutants, catalytic fragment corresponding to the last C-terminal 250 amino acids Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.37 2'-AMP product inhibitor Rattus norvegicus
3.1.4.37 5,5'-dithiobis-(2-nitrobenzoic acid) 97% inhibition of the catalytic fragment of CNP1 in a time- and dose-dependent manner, kinetics, fully reversed by excess dithiothreitol or 2-mercaptoethanol, inhibition is attributable to steric effects of modification of Cys-236 and Cys-314 by the inhibitor Rattus norvegicus
3.1.4.37 diethylpyrocarbonate pH 6.5, 22°C, time-dependent inhibition, kinetics, completely reversed by 0.5 M hydroxylamine Rattus norvegicus
3.1.4.37 KCN 62% inhibition of the catalytic fragment of CNP1 Rattus norvegicus
3.1.4.37 methyl methanethiosulfonate 42% inhibition of the catalytic fragment of CNP1 Rattus norvegicus
3.1.4.37 additional information not inhibited by dithiothreitol Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.37 0.098
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
3.1.4.37 0.1
-
 2',3'-cNADP pH 6, 25°C, H309F mutant CNP1 Rattus norvegicus
3.1.4.37 0.104
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
3.1.4.37 0.119
-
 2',3'-cNADP pH 6, 25°C, H230F mutant CNP1 Rattus norvegicus
3.1.4.37 0.231
-
 2',3'-cNADP pH 6, 25°C, C231A mutant CNP1 Rattus norvegicus
3.1.4.37 0.237
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length CNP1 Rattus norvegicus
3.1.4.37 0.241
-
 2',3'-cNADP pH 6, 25°C, C314S mutant CNP1 Rattus norvegicus
3.1.4.37 0.295
-
 2',3'-cNADP pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids Rattus norvegicus
3.1.4.37 0.333
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
3.1.4.37 0.354
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
3.1.4.37 0.379
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
3.1.4.37 0.473
-
 2',3'-cNADP pH 6, 25°C, C231S mutant CNP1 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.37 46000
-
 x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus
3.1.4.37 48000
-
 x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.37 additional information Rattus norvegicus CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.4.37 Ethanol at a final concentration up to 4% v/v of the total volume, no effect on CNP1 activity Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.37 Rattus norvegicus
-
 2 isoforms: CNP1 and CNP2 differing by a 20-amino acid extension at the N-terminus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.37 side-chain modification isoprenylated at Cys-397 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.37 recombinant wild-type and mutant CNP1 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.37 2',3'-cNADP+ + H2O
-
 Rattus norvegicus NADP+
-
 ?
3.1.4.37 2',3'-cyclic NADP+ + H2O
-
 Rattus norvegicus NADP+
-
 ?
3.1.4.37 additional information CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination Rattus norvegicus ?
-
 ?
3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O His-230 and His-309 of CNP1 play critical roles in enzyme catalysis, no essential role of cysteines, the catalytic domain forms a compact globular structure Rattus norvegicus nucleoside 2'-phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
3.1.4.37 ? x * 46000, CNP1, x * 48000, CNP2 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.37 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.37 1.15
-
 2',3'-cNADP pH 6, 25°C, H230F mutant CNP1 Rattus norvegicus
3.1.4.37 1.16
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
3.1.4.37 1.27
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
3.1.4.37 1.35
-
 2',3'-cNADP pH 6, 25°C, H309F mutant CNP1 Rattus norvegicus
3.1.4.37 6.08
-
 2',3'-cNADP pH 6, 25°C, H230L mutant CNP1 Rattus norvegicus
3.1.4.37 6.08
-
 2',3'-cNADP pH 6, 25°C, H309L mutant CNP1 Rattus norvegicus
3.1.4.37 594
-
 2',3'-cNADP pH 6, 25°C, C314S mutant CNP1 Rattus norvegicus
3.1.4.37 825
-
 2',3'-cNADP pH 6, 25°C, C231S mutant CNP1 Rattus norvegicus
3.1.4.37 968
-
 2',3'-cNADP pH 6, 25°C, C231A mutant CNP1 Rattus norvegicus
3.1.4.37 1107
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
3.1.4.37 1110
-
 2',3'-cNADP pH 6, 25°C, C236S mutant CNP1 Rattus norvegicus
3.1.4.37 1116
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
3.1.4.37 1120
-
 2',3'-cNADP pH 6, 25°C, C314A mutant CNP1 Rattus norvegicus
3.1.4.37 1130
-
 2',3'-cNADP pH 6, 25°C, C397S mutant CNP1 Rattus norvegicus
3.1.4.37 1132
-
 2',3'-cNADP pH 6, 25°C, C397S mutant CNP1 Rattus norvegicus
3.1.4.37 1195
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length wild-type CNP1 Rattus norvegicus
3.1.4.37 1200
-
 2',3'-cNADP pH 6, 25°C, recombinant full-length wild-type CNP1 Rattus norvegicus
3.1.4.37 1476
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
3.1.4.37 1480
-
 2',3'-cNADP pH 6, 25°C, C236A mutant CNP1 Rattus norvegicus
3.1.4.37 1690
-
 2',3'-cNADP pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.37 6
-
 assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.37 0.5
-
 2'-AMP
-
 Rattus norvegicus