BRENDA - Enzyme Database show

Substrate binding is the rate-limiting step in thromboxane synthase catalysis

Wang, L.H.; Tsai, A.L.; Hsu, P.Y.; J. Biol. Chem. 276, 14737-14743 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.5
expression in Escherichia coli
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.021
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
recombinant TXAS
Homo sapiens
5.3.99.5
0.022
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
native TXAS
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
endoplasmic reticulum
-
Homo sapiens
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
Homo sapiens
?
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
12-L-hydroxy-5,8,10-heptadecaotrienoic acid + malondialdehyde
ratio of TXA2/heptadecatrienoic acid/malondialdehyde, 1/1/1
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.5
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.5
recombinant His-tagged TXAS
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652105
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
652105
Homo sapiens
?
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652105
Homo sapiens
12-L-hydroxy-5,8,10-heptadecaotrienoic acid + malondialdehyde
ratio of TXA2/heptadecatrienoic acid/malondialdehyde, 1/1/1
652105
Homo sapiens
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.017
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
recombinant TXAS
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
expression in Escherichia coli
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.021
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
recombinant TXAS
Homo sapiens
5.3.99.5
0.022
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
native TXAS
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
endoplasmic reticulum
-
Homo sapiens
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
Homo sapiens
?
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
12-L-hydroxy-5,8,10-heptadecaotrienoic acid + malondialdehyde
ratio of TXA2/heptadecatrienoic acid/malondialdehyde, 1/1/1
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
recombinant His-tagged TXAS
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652105
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
652105
Homo sapiens
?
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652105
Homo sapiens
12-L-hydroxy-5,8,10-heptadecaotrienoic acid + malondialdehyde
ratio of TXA2/heptadecatrienoic acid/malondialdehyde, 1/1/1
652105
Homo sapiens
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.5
0.017
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
recombinant TXAS
Homo sapiens