BRENDA - Enzyme Database

Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance

Hunsucker, S.A.; Spychala, J.; Mitchell, B.S.; J. Biol. Chem. 276, 10498-10504 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
required for full activity, of the recombinant human enzyme, allosteric activator
Homo sapiens
3.1.3.5
ATP
activates
Homo sapiens
3.1.3.5
GTP
serves as activitor, not as strong as ADP
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.5
expression in Sf9 insect cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
2-fluoroadenine-9-beta-D-arabinofuranoside
1 mM, 70% inhibition with 5 mM 5'-AMP as substrate
Homo sapiens
3.1.3.5
araCMP
1 mM, 25% inhibition with 5 mM 5'-AMP as substrate
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.012
-
dCMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
0.193
-
dCMP
pH 7.0, 37°C, without ADP
Homo sapiens
3.1.3.5
1.87
-
5'-AMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
2.47
-
5'-IMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
2.96
-
5'-AMP
pH 7.0, 37°C, without ADP
Homo sapiens
3.1.3.5
12.11
-
5'-IMP
pH 7.0, 37°C, without ADP
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
47700
-
x * 47700, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
additional information
Homo sapiens
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
?
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Homo sapiens
Q9BXI3
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
brain
-
Homo sapiens
-
3.1.3.5
heart
high activity
Homo sapiens
-
3.1.3.5
HEK-293 cell
-
Homo sapiens
-
3.1.3.5
JURKAT cell
-
Homo sapiens
-
3.1.3.5
kidney
low activity
Homo sapiens
-
3.1.3.5
pancreas
-
Homo sapiens
-
3.1.3.5
skeletal muscle
high activity
Homo sapiens
-
3.1.3.5
testis
low activity
Homo sapiens
-
3.1.3.5
uterus
low activity
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
14.4
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
2-chloro-2'-deoxyadenosine monophosphate + H2O
39% of the activity with 5'-AMP
652095
Homo sapiens
2-chloro-2'-deoxyadenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-AMP + H2O
-
652095
Homo sapiens
adenosine + phosphate
-
-
-
-
3.1.3.5
5'-AMP + H2O
-
652095
Homo sapiens
adenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
85% of the activity with 5'-AMP
652095
Homo sapiens
cytidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-dIMP + H2O
61% of the activity with 5'-AMP
652095
Homo sapiens
deoxyinosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
57% of the activity with 5'-AMP
652095
Homo sapiens
guanosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-IMP + H2O
-
652095
Homo sapiens
inosine + phosphate
-
-
-
-
3.1.3.5
5'-TMP + H2O
13% of the activity with 5'-AMP
652095
Homo sapiens
thymidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-UMP + H2O
78% of the activity with 5'-AMP
652095
Homo sapiens
uridine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dAMP + H2O
91% of the activity with 5'-AMP
652095
Homo sapiens
deoxyadenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dCMP + H2O
10% of the activity with 5'-AMP
652095
Homo sapiens
deoxycytidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dGMP + H2O
75% of the activity with 5'-AMP
652095
Homo sapiens
deoxyguanosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dIMP + H2O
84% of the activity with 5'-AMP
652095
Homo sapiens
deoxyinosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dUMP + H2O
7% of the activity with 5'-AMP
652095
Homo sapiens
deoxyuridine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
additional information
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
652095
Homo sapiens
?
-
652095
Homo sapiens
?
3.1.3.5
additional information
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
652095
Homo sapiens
?
-
652095
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 47700, calculation from nucleotide sequence
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
required for full activity, of the recombinant human enzyme, allosteric activator
Homo sapiens
3.1.3.5
ATP
activates
Homo sapiens
3.1.3.5
GTP
serves as activitor, not as strong as ADP
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
expression in Sf9 insect cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
2-fluoroadenine-9-beta-D-arabinofuranoside
1 mM, 70% inhibition with 5 mM 5'-AMP as substrate
Homo sapiens
3.1.3.5
araCMP
1 mM, 25% inhibition with 5 mM 5'-AMP as substrate
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.012
-
dCMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
0.193
-
dCMP
pH 7.0, 37°C, without ADP
Homo sapiens
3.1.3.5
1.87
-
5'-AMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
2.47
-
5'-IMP
pH 7.0, 37°C, in presence of 1 mM ADP
Homo sapiens
3.1.3.5
2.96
-
5'-AMP
pH 7.0, 37°C, without ADP
Homo sapiens
3.1.3.5
12.11
-
5'-IMP
pH 7.0, 37°C, without ADP
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
47700
-
x * 47700, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
additional information
Homo sapiens
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
?
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
brain
-
Homo sapiens
-
3.1.3.5
heart
high activity
Homo sapiens
-
3.1.3.5
HEK-293 cell
-
Homo sapiens
-
3.1.3.5
JURKAT cell
-
Homo sapiens
-
3.1.3.5
kidney
low activity
Homo sapiens
-
3.1.3.5
pancreas
-
Homo sapiens
-
3.1.3.5
skeletal muscle
high activity
Homo sapiens
-
3.1.3.5
testis
low activity
Homo sapiens
-
3.1.3.5
uterus
low activity
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
14.4
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
2-chloro-2'-deoxyadenosine monophosphate + H2O
39% of the activity with 5'-AMP
652095
Homo sapiens
2-chloro-2'-deoxyadenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-AMP + H2O
-
652095
Homo sapiens
adenosine + phosphate
-
-
-
-
3.1.3.5
5'-AMP + H2O
-
652095
Homo sapiens
adenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
85% of the activity with 5'-AMP
652095
Homo sapiens
cytidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-dIMP + H2O
61% of the activity with 5'-AMP
652095
Homo sapiens
deoxyinosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
57% of the activity with 5'-AMP
652095
Homo sapiens
guanosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-IMP + H2O
-
652095
Homo sapiens
inosine + phosphate
-
-
-
-
3.1.3.5
5'-TMP + H2O
13% of the activity with 5'-AMP
652095
Homo sapiens
thymidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
5'-UMP + H2O
78% of the activity with 5'-AMP
652095
Homo sapiens
uridine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dAMP + H2O
91% of the activity with 5'-AMP
652095
Homo sapiens
deoxyadenosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dCMP + H2O
10% of the activity with 5'-AMP
652095
Homo sapiens
deoxycytidine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dGMP + H2O
75% of the activity with 5'-AMP
652095
Homo sapiens
deoxyguanosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dIMP + H2O
84% of the activity with 5'-AMP
652095
Homo sapiens
deoxyinosine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
dUMP + H2O
7% of the activity with 5'-AMP
652095
Homo sapiens
deoxyuridine + phosphate
-
652095
Homo sapiens
?
3.1.3.5
additional information
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
652095
Homo sapiens
?
-
652095
Homo sapiens
?
3.1.3.5
additional information
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
652095
Homo sapiens
?
-
652095
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 47700, calculation from nucleotide sequence
Homo sapiens