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Literature summary extracted from

  • Bernardo, K.; Krut, O.; Wiegmann, K.; Kreder, D.; Micheli, M.; Schafer, R.; Sickman, A.; Schmidt, W.E.; Schroder, J.M.; Meyer, H.E.; Sandhoff, K.; Kronke, M.
    Purification and characterization of a magnesium-dependent neutral sphingomyelinase from bovine brain (2000), J. Biol. Chem., 275, 7641-7647.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 phosphatidylcholine 0.2 mM, 2fold activation Bos taurus
3.1.4.12 phosphatidylethanolamine 0.2 mM, 2.9fold activation Bos taurus
3.1.4.12 phosphatidylserine 0.2 mM, 3.8fold activation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Ca2+ 0.1 mM, 60% inhibition of Mg2+-activated N-SMase Bos taurus
3.1.4.12 EDTA 10 mM, 95% inhibition Bos taurus
3.1.4.12 glutathione 0.2 mM, almost complete inhibition Bos taurus
3.1.4.12 scyphostatin 0.02 mM, complete inhibition Bos taurus
3.1.4.12 Zn2+ almost complete inhibition of Mg2+-activated N-SMase Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.04
-
 sphingomyelin 37°C, pH 7.4 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 plasma membrane associated Bos taurus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ required for activity, approx. 3fold activation at 0.1-0.5 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 97000
-
 x * 97000, 2D-electrophoresis Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Bos taurus
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 NP-40, CM-Sepharose, concanavalin A-Sepharose, Methyl-macroprep, isoelectric focusing Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 0.23
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Bos taurus N-acylsphingosine + choline phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 ? x * 97000, 2D-electrophoresis Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 N-SMase
-
 Bos taurus
3.1.4.12 neutral sphingomyelinase
-
 Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.12 56
-
 95% loss of activity after 5 min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7 8
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.12 6 9 approx. 30% of maximal activity at pH 6.5, approx. 70% of maximal activity at pH 9.0 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.12 Bos taurus isoelectric focusing
-
 5.3