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Literature summary extracted from

  • Weyand, M.; Schlichting, I.
    Structural basis for the impaired channeling and allosteric inter-subunit communication in the beta A169L/beta C170W mutant of tryptophan synthase (2000), J. Biol. Chem., 275, 41058-41063.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.20 purified mutant A169L/C170W complexed with the alpha-active site substrate analogue 5-fluoro-indole-propanol-phosphate, X-ray diffraction structure determination and analysis at 2.25 A resolution Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 A169L/C170W mutants of beta-subunit residues, altered kinetic properties and conformation Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 additional information
-
 additional information kinetics of wild-type enzyme complex and mutant betaA169L/betaC170W Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bifunctional enzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bifunctional enzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 additional information substrate channeling, Thr170 occludes the tunnel connecting the alpha- and beta-active sites, accumulation of the intermediate indole during a single enzyme turnover Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 tetramer alpha2beta2 complex, structural organization Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 TRPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium