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Literature summary extracted from

  • Komori, K.; Sakae, S.; Daiyasu, H.; Toh, H.; Morikawa, K.; Shinagawa, H.; Ishino, Y.
    Mutational analysis of the Pyrococcus furiosus holliday junction resolvase hjc revealed functionally important residues for dimer formation, junction DNA binding, and cleavage activities (2000), J. Biol. Chem., 275, 40385-40391.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.10 D33A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 E110A mutant enzyme is as active as the wild-type enzyme Pyrococcus furiosus
3.1.21.10 E11A mutant enzyme is as active as the wild-type enzyme Pyrococcus furiosus
3.1.21.10 E46A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 E9A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 F21A mutant enzyme is as active as the wild-type enzyme Pyrococcus furiosus
3.1.21.10 F68A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 F72A no cleavage of Holliday junction. Mutant enzyme exists as monomer more frequently in solution than as dimer Pyrococcus furiosus
3.1.21.10 F89A mutant enzyme is as active as the wild-type enzyme Pyrococcus furiosus
3.1.21.10 K48A no cleavage of Holliday junction. Mutant enzyme exists as monomer more frequently in solution than as dimer Pyrococcus furiosus
3.1.21.10 K81A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 R10A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 R25A no cleavage of Holliday junction Pyrococcus furiosus
3.1.21.10 Y56A some decrease in activity Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.10 14300
-
 2 * 14300, wild-type enzyme, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Pyrococcus furiosus
-
-
-

Subunits

EC Number Subunits Comment Organism
3.1.21.10 dimer 2 * 14300, wild-type enzyme, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.10 holliday junction resolvase hjc
-
 Pyrococcus furiosus