Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kast, P.; Grisostomi, C.; Chen, I.A.; Li, S.; Krengel, U.; Xue, Y.; Hilvert, D.
    A strategically positioned cation is crucial for efficient catalysis by chorismate mutase (2000), J. Biol. Chem., 275, 36832-36838.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 overexpression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.5 crystal structures of double mutants C88S/R90K and C88K/R90S, hanging drop vapour diffusion method at room temperature, space group R3 with a and b: 82.6 A and c: 42.8 A Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 C88K/R90S lower activity than wild-type enzyme Bacillus subtilis
5.4.99.5 C88S/R90K lower activity than wild-type enzyme Bacillus subtilis
5.4.99.5 R90A no activity detectable Bacillus subtilis
5.4.99.5 R90G very low activity Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.5 oxabicyclic dicarboxylic acid transition state analogon, competitive inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.067
-
chorismate 30°C, pH 7, wild-type Bacillus subtilis
5.4.99.5 0.15
-
chorismate 30°C, pH 7, mutant R90G Bacillus subtilis
5.4.99.5 1.9
-
chorismate 30°C, pH 7, mutant C88K/R90S Bacillus subtilis
5.4.99.5 4.3
-
chorismate 30°C, pH 7, mutant C88S/R90K Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Bacillus subtilis first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants Prephenate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Bacillus subtilis P19080
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
Bacillus subtilis Prephenate
-
?
5.4.99.5 Chorismate first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants Bacillus subtilis Prephenate
-
?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 BsCM
-
Bacillus subtilis
5.4.99.5 chorismate mutase
-
Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 0.0003
-
chorismate 30°C, pH 7, mutant R90G Bacillus subtilis
5.4.99.5 0.29
-
chorismate 30°C, pH 7, mutant C88S/R90K Bacillus subtilis
5.4.99.5 0.32
-
chorismate 30°C, pH 7, mutant C88K/R90S Bacillus subtilis
5.4.99.5 46
-
chorismate 30°C, pH 7, wild-type Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.99.5 additional information
-
oxabicyclic dicarboxylic acid >> 1 mM mutant C88S/R90K Bacillus subtilis
5.4.99.5 0.003
-
oxabicyclic dicarboxylic acid 30°C, pH 7, wild-type Bacillus subtilis
5.4.99.5 0.004
-
oxabicyclic dicarboxylic acid 30°C, pH 7, mutant R90G Bacillus subtilis
5.4.99.5 1.1
-
oxabicyclic dicarboxylic acid 30°C, pH 7, mutant C88K/R90S Bacillus subtilis