BRENDA - Enzyme Database show

Structural basis of hematopoietic prostaglandin D synthase activity elucidated by site-directed mutagenesis

Pinzar, E.; Miyano, M.; Kanaoka, Y.; Urade, Y.; Hayaishi, O.; J. Biol. Chem. 275, 31239-31244 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
-
Rattus norvegicus
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.2
-
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
C156L
loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
C156Y
loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
K112E
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
K198E
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
L199F
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
R14E
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
R14K
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
W104I
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
Y152F
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
Y8F
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.1
-
glutathione
-
Rattus norvegicus
5.3.99.2
0.5
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
5.3.99.2
0.5
-
glutathione
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
3
-
1-chloro-2,4-dinitrobenzene
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Rattus norvegicus
O35543
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.2
30
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652044
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
652044
Rattus norvegicus
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH
-
652044
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
652044
Rattus norvegicus
?
5.3.99.2
glutathione + 1-chloro-2,4-dinitrobenzene
-
652044
Rattus norvegicus
?
-
652044
Rattus norvegicus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.2
2
-
glutathione
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
5
-
1-chloro-2,4-dinitrobenzene
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
15
-
glutathione
-
Rattus norvegicus
5.3.99.2
21.7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Rattus norvegicus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.2
-
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
C156L
loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
C156Y
loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
K112E
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
K198E
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
L199F
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
R14E
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
R14K
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
W104I
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
Y152F
retention of prostaglandin D synthase and glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
Y8F
complete loss of prostaglandin D synthase activity and glutathione S-transferase activity
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.1
-
glutathione
-
Rattus norvegicus
5.3.99.2
0.5
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
5.3.99.2
0.5
-
glutathione
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
3
-
1-chloro-2,4-dinitrobenzene
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
Rattus norvegicus
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.2
30
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
652044
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
652044
Rattus norvegicus
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH
-
652044
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
652044
Rattus norvegicus
?
5.3.99.2
glutathione + 1-chloro-2,4-dinitrobenzene
-
652044
Rattus norvegicus
?
-
652044
Rattus norvegicus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.2
2
-
glutathione
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
5
-
1-chloro-2,4-dinitrobenzene
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene
Rattus norvegicus
5.3.99.2
15
-
glutathione
-
Rattus norvegicus
5.3.99.2
21.7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus