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Literature summary extracted from

  • Ro, H.S.; Miles, E.W.
    Structure and function of the tryptophan synthase alpha(2)beta(2) complex. Roles of beta subunit histidine 86 (1999), J. Biol. Chem., 274, 36439-36445.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 H86L site-directed mutagenesis, exchange of His86, located adjacent to beta subunit Lys87, which forms an intermediate with the pyridoxal 5'-phosphate, leads to 20fold reduced binding of the cofactor and reduced formation the reaction intermediate, altered pH-profile Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 additional information
-
 additional information kinetics, wild-type and mutant H86L enzymes Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism and intermediates Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 tetramer alpha2beta2 complex, structure-function relationship Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.20 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.20 7.5
-
 wild-type Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 8.8
-
 mutant H86L Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate dependent on, His86 is necessary for binding and stabilization in a correct orientation in the active site of the beta-subunit Salmonella enterica subsp. enterica serovar Typhimurium