EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.34 | DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21, stable expression in 293 cells and secretion to the medium | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 46000 | - |
x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE | Homo sapiens |
3.4.22.34 | 50000 | - |
x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | additional information | Homo sapiens | enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone | ? | - |
? | |
3.4.22.34 | protein + H2O | Homo sapiens | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.34 | Homo sapiens | Q99538 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.34 | recombinant His-tagged enzyme from Escherichia coli | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.34 | bone marrow | plasma | Homo sapiens | - |
3.4.22.34 | additional information | tissue distribution, low expression level in all tissues tested, no expression in MG63 osteosarcoma cells | Homo sapiens | - |
3.4.22.34 | osteoblast | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | additional information | enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone | Homo sapiens | ? | - |
? | |
3.4.22.34 | protein + H2O | - |
Homo sapiens | peptides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.34 | ? | x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.34 | Asparaginyl endopeptidase | - |
Homo sapiens |