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Literature summary extracted from

  • Choi, S.J.; Reddy, S.V.; Devlin, R.D.; Menaa, C.; Chung, H.; Boyce, B.B.; Roodman, G.D.
    Identification of human asparaginyl endopeptidase (legumain) as an inhibitor of osteoclast formation and bone resorption (1999), J. Biol. Chem., 274, 27747-27753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21, stable expression in 293 cells and secretion to the medium Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 46000
-
 x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens
3.4.22.34 50000
-
 x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 additional information Homo sapiens enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone ?
-
 ?
3.4.22.34 protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens Q99538
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 recombinant His-tagged enzyme from Escherichia coli Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 bone marrow plasma Homo sapiens
-
3.4.22.34 additional information tissue distribution, low expression level in all tissues tested, no expression in MG63 osteosarcoma cells Homo sapiens
-
3.4.22.34 osteoblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 additional information enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone Homo sapiens ?
-
 ?
3.4.22.34 protein + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Asparaginyl endopeptidase
-
 Homo sapiens