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Literature summary extracted from

  • Cheng, D.; Espenshade, P.J.; Slaughter, C.A.; Jaen, J.C.; Brown, M.S.; Goldstein, J.L.
    Secreted site-1 protease cleaves peptides corresponding to luminal loop of sterol regulatory element-binding proteins (1999), J. Biol. Chem., 274, 22805-22812.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.112 truncated from lacking the COOH-terminal membrane anchor, expressed in CHO cells Mammalia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.112 1,10-phenanthroline partial inhibition at very high concentrations Mammalia
3.4.21.112 Ca2+ inhibition above 3 mM Mammalia
3.4.21.112 dithiothreitol partial inhibition at very high concentrations Mammalia
3.4.21.112 EDTA partial inhibition at very high concentrations Mammalia
3.4.21.112 EGTA partial inhibition at very high concentrations Mammalia
3.4.21.112 Pefabloc SC at high concentrations Mammalia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.112 0.1
-
 Ac-VFRSLK-4-methyl-coumaryl-7-amide pH 8, 37°C Mammalia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.112 additional information Mammalia functions to control lipid biosynthesis and uptake in animal cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.112 Mammalia
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.112 proteolytic modification enzyme undergoes autocatalytic processing, generating an active form S1P-B, which is further cleaved after residue 186 to generate S1P-C Mammalia

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.112
-
 Mammalia

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.112 Ac-VFRSLK-4-methyl-coumaryl-7-amide + H2O
-
 Mammalia ?
-
 ?
3.4.21.112 Ac-VFRSLK-4-methylcoumarin 7-amide
-
 Mammalia ?
-
 ?
3.4.21.112 additional information cleaves after lysine or leucine rather than arginine Mammalia ?
-
 ?
3.4.21.112 additional information functions to control lipid biosynthesis and uptake in animal cells Mammalia ?
-
 ?
3.4.21.112 sterol regulatory element-binding protein + H2O SREBP Mammalia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.112 S1P
-
 Mammalia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.112 7 8
-
 Mammalia