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Literature summary extracted from

  • Krappmann, S.; Helmstaedt, K.; Gerstberger, T.; Eckert, S.; Hoffmann, B.; Hoppert, M.; Schnappauf, G.; Braus, G.H.
    The aroC gene of Aspergillus nidulans codes for a monofunctional, allosterically regulated chorismate mutase (1999), J. Biol. Chem., 274, 22275-22282.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.5 tryptophan 0.005 mM, heterotrophic activator Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 overexpression in a Saccharomyces cerevisiae aro7DELTA mutant strain Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 D233I little reduced regulatory range through tyrosine and tryptophan than wild-type enzyme Aspergillus nidulans
5.4.99.5 D233T strong reduced regulatory range through tyrosine and tryptophan than wild-type enzyme Aspergillus nidulans
5.4.99.5 T226D reduced regulatory range through tyrosine and tryptophan than wild-type enzyme Saccharomyces cerevisiae
5.4.99.5 T226I reduced regulatory range through tyrosine and tryptophan than wild-type enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.5 chorismate
-
 Aspergillus nidulans
5.4.99.5 tyrosine 0.05 mM, heterotrophic feedback-inhibitor Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 30000
-
 alpha2, 2 * 30000, calculated from amino acid sequence, homology modeling, electron microscopy Aspergillus nidulans
5.4.99.5 62200
-
 gel filtration Aspergillus nidulans
5.4.99.5 65000
-
 PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Saccharomyces cerevisiae enzyme of the first branch point of aromatic amino acid biosynthesis Prephenate
-
 ?
5.4.99.5 Chorismate Aspergillus nidulans enzyme of the first branch point of aromatic amino acid biosynthesis Prephenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Aspergillus nidulans Q9Y7B2
-
-
5.4.99.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
 Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.5 1200
-
 0.1 mM tyrosine, wild-type Saccharomyces cerevisiae
5.4.99.5 3300
-
 0.1 mM tyrosine, mutant T226D Saccharomyces cerevisiae
5.4.99.5 3700
-
 unliganded, mutant T226D Saccharomyces cerevisiae
5.4.99.5 4800
-
 unliganded, wild-type Saccharomyces cerevisiae
5.4.99.5 8400
-
 0.1 mM tyrosine, wild-type Aspergillus nidulans
5.4.99.5 11100
-
 0.5 mM tryptophan, mutant T226D Saccharomyces cerevisiae
5.4.99.5 13400
-
 0.1 mM tyrosine, mutant D233I Aspergillus nidulans
5.4.99.5 20600
-
 unliganded, mutant T226I Saccharomyces cerevisiae
5.4.99.5 21700
-
 0.1 mM tyrosine, mutant T226I Saccharomyces cerevisiae
5.4.99.5 22000
-
 0.1 mM tyrosine, mutant D233T Aspergillus nidulans
5.4.99.5 26400
-
 0.5 mM tryptophan, mutant T226I Saccharomyces cerevisiae
5.4.99.5 30300
-
 unliganded, mutant D233I Aspergillus nidulans
5.4.99.5 32500
-
 unliganded, wild-type Aspergillus nidulans
5.4.99.5 40200
-
 0.5 mM tryptophan, wild-type Saccharomyces cerevisiae
5.4.99.5 41300
-
 unliganded, mutant D233T Aspergillus nidulans
5.4.99.5 63000
-
 0.5 mM tryptophan, mutant D233I Aspergillus nidulans
5.4.99.5 64300
-
 0.5 mM tryptophan, mutant D233T Aspergillus nidulans
5.4.99.5 88500
-
 0.5 mM tryptophan, wild-type Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Saccharomyces cerevisiae Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Aspergillus nidulans Prephenate
-
 ?
5.4.99.5 Chorismate enzyme of the first branch point of aromatic amino acid biosynthesis Saccharomyces cerevisiae Prephenate
-
 ?
5.4.99.5 Chorismate enzyme of the first branch point of aromatic amino acid biosynthesis Aspergillus nidulans Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 homodimer alpha2, 2 * 30000, calculated from amino acid sequence, homology modeling, electron microscopy Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 chorismate mutase
-
 Saccharomyces cerevisiae
5.4.99.5 chorismate mutase
-
 Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 82
-
 chorismate 30°C, pH 7.6 Aspergillus nidulans
5.4.99.5 92
-
 chorismate 30°C, pH 7.6, in presence of 0.005 mM tryptophan Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.5 5.4
-
 catalytic maximum in presence of tyrosine Aspergillus nidulans
5.4.99.5 7.1
-
 catalytic maximum in presence of tryptophan Aspergillus nidulans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.99.5 0.0028
-
 chorismate 30°C, pH 7.6, in presence of 0.005 mM tyrosine Aspergillus nidulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.4.99.5 Aspergillus nidulans isoelectric focusing, pH-gradient 3.5-9.5 4.8 4.7