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Literature summary extracted from

  • Alvarez-Fernandez, M.; Barrett, A.J.; Gerhartz, B.; Dando, P.M.; Ni, J.; Abrahamson, M.
    Inhibition of mammalian legumain by some cystatins is due too a novel second reactive site (1999), J. Biol. Chem., 274, 19195-19203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 Human cystatin C competitive inhibition Sus scrofa
3.4.22.34 human cystatin C mutant L9G inhibitory potential similar to wild-type cystatin C Sus scrofa
3.4.22.34 human cystatin C mutant N39K reduced inhibitory effect compared to the wild-type cystatin C Sus scrofa
3.4.22.34 human cystatin C mutant R8G inhibitory potential similar to wild-type cystatin C Sus scrofa
3.4.22.34 human cystatin C mutant V10G inhibitory potential similar to wild-type cystatin C Sus scrofa
3.4.22.34 human cystatin C mutant W106G inhibitory potential similar to wild-type cystatin C Sus scrofa
3.4.22.34 human cystatin E/M type 2 cystatin Sus scrofa
3.4.22.34 human cystatin F type 2 cystatin Sus scrofa
3.4.22.34 additional information inhibition mechanism by human cystatins, enzyme inhibitor complex formation analysis, no inhibition by human type 1 cystatins A and B, type 2 cystatin D, and type 3 low MW kininogen Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 33100
-
 1 * 33100, glycosylated native enzyme, SDS-PAGE Sus scrofa
3.4.22.34 35300
-
 glycosylated native enzyme, gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 protein + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Sus scrofa
-
 purified enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 benzoyl-Asn-4-nitroanilide + H2O
-
 Sus scrofa benzoyl-Asn + 4-nitroaniline
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 protein + H2O
-
 Sus scrofa peptides
-
 ?
3.4.22.34 protein + H2O an Asn residue at P1 position is strictly required Sus scrofa peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 monomer 1 * 33100, glycosylated native enzyme, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.6
-
 assay at Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.34 0.0000000016
-
 human cystatin E/M pH 5.6, 37°C Sus scrofa
3.4.22.34 0.0000002
-
 Human cystatin C pH 5.6, 37°C Sus scrofa
3.4.22.34 0.00000022
-
 human cystatin C mutant R8G pH 5.6, 37°C Sus scrofa
3.4.22.34 0.00000022
-
 human cystatin C mutant L9G pH 5.6, 37°C Sus scrofa
3.4.22.34 0.00000022
-
 human cystatin C mutant V10G pH 5.6, 37°C Sus scrofa
3.4.22.34 0.00000022
-
 human cystatin C mutant W106G pH 5.6, 37°C Sus scrofa
3.4.22.34 0.00001
-
 human cystatin F pH 5.6, 37°C Sus scrofa
3.4.22.34 0.001
-
 human cystatin C mutant N39K above, pH 5.6, 37°C Sus scrofa