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Literature summary extracted from
- Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides (1998), J. Biol. Chem., 273, 30225-30231.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.B16 | extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.B16 | 20000 | - |
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE | Pseudomonas aeruginosa |
| 3.4.24.B16 | 42000 | - |
x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B16 | protein + H2O | Pseudomonas aeruginosa | - |
peptides | - |
? |  |
| 3.4.24.B16 | protein + H2O | Pseudomonas aeruginosa FRD740 | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.B16 | Pseudomonas aeruginosa | - |
- |
- |
| 3.4.24.B16 | Pseudomonas aeruginosa FRD740 | - |
- |
- |
| 3.4.24.26 | Pseudomonas aeruginosa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.B16 | proteolytic modification | no autoproteolysis, enzyme is synthesized as a pre-proLasA, the signal sequence of pre-proLasA is cleaved off by a signal peptidase, the remaining inactive 42 kDa proLasA is secreted to the extracellular space, where the proform is cleaved to the mature active enzyme via a 28 kDa intermediate, plus a 14 kDa fragment, by elastase and a lysine specific protease, not by alkaline proteinase | Pseudomonas aeruginosa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.B16 | from culture supernatant | Pseudomonas aeruginosa |
| 3.4.24.26 | - |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B16 | protein + H2O | - |
Pseudomonas aeruginosa | peptides | - |
? | |
| 3.4.24.B16 | protein + H2O | - |
Pseudomonas aeruginosa FRD740 | peptides | - |
? | |
| 3.4.24.26 | elastase propeptide + H2O | autocatalytically cleaved | Pseudomonas aeruginosa | elastase + ? | - |
? | |
| 3.4.24.26 | elastase propeptide + H2O | autocatalytically cleaved | Pseudomonas aeruginosa FRD740 | elastase + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.B16 | ? | x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.B16 | M23.002 | Merops-ID | Pseudomonas aeruginosa |
| 3.4.24.B16 | staphylolysin | - |
Pseudomonas aeruginosa |
| 3.4.24.26 | elastase | - |
Pseudomonas aeruginosa |
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