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Literature summary extracted from

  • Engel, L.S.; Hill, J.M.; Caballero, A.R.; Green, L.C.; O'Callaghan, R.J.
    Protease IV, a unique extracellular protease and virulence factor from Pseudomonas aeruginosa (1998), J. Biol. Chem., 273, 16792-16797.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.89 Aprotinin
-
Pseudomonas aeruginosa
3.4.21.89 bestatin
-
Pseudomonas aeruginosa
3.4.21.89 EDTA
-
Pseudomonas aeruginosa
3.4.21.89 EGTA
-
Pseudomonas aeruginosa
3.4.21.89 Elastinal
-
Pseudomonas aeruginosa

Application

EC Number Application Comment Organism
3.4.21.89 medicine developing of medication designed to arrest tissue damage during Pseudomonas infection, opportunistic pathogen causes morbidity and mortality in patients with burns, cystic fibrosis, pneumonia, urinary tract infections, skin infections, cancer, acquired immunodeficiency syndrome, and ocular disease Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.89
-
Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.89 beta-mercaptoethanol
-
Pseudomonas aeruginosa
3.4.21.89 diisopropyl fluorophosphate partial inhibition Pseudomonas aeruginosa
3.4.21.89 dithiothreitol
-
Pseudomonas aeruginosa
3.4.21.89 additional information not inhibited by thiol-, carboxyl-, or metalloproteinase inhibitors Pseudomonas aeruginosa
3.4.21.89 N-[(1S)-1-(chloroacetyl)-3-methylbutyl]-4-methylbenzenesulfonamide total loss of enzyme activity Pseudomonas aeruginosa
3.4.21.89 phenylmethyl sulfonyl fluoride partial inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.89 0.319
-
tosyl-Gly-Pro-Lys-p-nitroanilide pH 10.0, 25°C Pseudomonas aeruginosa
3.4.21.89 0.727
-
Val-Leu-Lys-p-nitroanilide pH 10.0, 25°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.89 extracellular
-
Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.89 26000
-
-
Pseudomonas aeruginosa
3.4.21.89 26380
-
mass spectrometry Pseudomonas aeruginosa
3.4.21.89 30000
-
gel filtration, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.89 signal peptides from preproteins + H2O Pseudomonas aeruginosa
-
mature proteins
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.89 Pseudomonas aeruginosa
-
-
-
3.4.21.89 Pseudomonas aeruginosa PA103-29
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.89
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.89 additional information
-
specific activity 471 units/mg, 1 unit is DELTA A/min x total assay volume/sample volume x E 410 nm x light path (cm) where the extinction coefficient E of the product p-nitroanilide at 410 nm is 9.75 and the light path is 0.53 cm Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.89 complement component C1q + H2O partially degraded Pseudomonas aeruginosa ?
-
?
3.4.21.89 Fibrinogen + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.21.89 Fibrinogen + H2O
-
Pseudomonas aeruginosa PA103-29 ?
-
?
3.4.21.89 IgG + H2O partially degraded Pseudomonas aeruginosa ?
-
?
3.4.21.89 Immunoglobulin + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.21.89 Immunoglobulin + H2O
-
Pseudomonas aeruginosa PA103-29 ?
-
?
3.4.21.89 additional information leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates Pseudomonas aeruginosa ?
-
?
3.4.21.89 additional information leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates Pseudomonas aeruginosa PA103-29 ?
-
?
3.4.21.89 plasmin + H2O partially degraded Pseudomonas aeruginosa ?
-
?
3.4.21.89 plasmin + H2O partially degraded Pseudomonas aeruginosa PA103-29 ?
-
?
3.4.21.89 plasminogen + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.21.89 signal peptides from preproteins + H2O
-
Pseudomonas aeruginosa mature proteins
-
?
3.4.21.89 tosyl-Gly-Pro-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa tosyl-Gly-Pro-Lys + p-nitroaniline
-
?
3.4.21.89 Val-Leu-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa Val-Leu-Lys + p-nitroaniline
-
?
3.4.21.89 Val-Leu-Lys-p-nitroanilide + H2O chromozym PL Pseudomonas aeruginosa PA103-29 Val-Leu-Lys + p-nitroaniline
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.89 monomer 1 * 30000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.4.21.89 protease IV
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.89 45
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.89 60 80 90% of the enzyme activity is abolished by incubation at 60°C for 40 min or 80°C for 1 min Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.89 10
-
-
Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.89 4 11 activity increases from pH 4.0-9.0, 85% of maximal activity at pH 11.0, no activity above pH 12.0 Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.89 Pseudomonas aeruginosa isoelectric focusing
-
8.7