EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.89 | Aprotinin | - |
Pseudomonas aeruginosa | |
3.4.21.89 | bestatin | - |
Pseudomonas aeruginosa | |
3.4.21.89 | EDTA | - |
Pseudomonas aeruginosa | |
3.4.21.89 | EGTA | - |
Pseudomonas aeruginosa | |
3.4.21.89 | Elastinal | - |
Pseudomonas aeruginosa |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.21.89 | medicine | developing of medication designed to arrest tissue damage during Pseudomonas infection, opportunistic pathogen causes morbidity and mortality in patients with burns, cystic fibrosis, pneumonia, urinary tract infections, skin infections, cancer, acquired immunodeficiency syndrome, and ocular disease | Pseudomonas aeruginosa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.89 | - |
Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.89 | beta-mercaptoethanol | - |
Pseudomonas aeruginosa | |
3.4.21.89 | diisopropyl fluorophosphate | partial inhibition | Pseudomonas aeruginosa | |
3.4.21.89 | dithiothreitol | - |
Pseudomonas aeruginosa | |
3.4.21.89 | additional information | not inhibited by thiol-, carboxyl-, or metalloproteinase inhibitors | Pseudomonas aeruginosa | |
3.4.21.89 | N-[(1S)-1-(chloroacetyl)-3-methylbutyl]-4-methylbenzenesulfonamide | total loss of enzyme activity | Pseudomonas aeruginosa | |
3.4.21.89 | phenylmethyl sulfonyl fluoride | partial inhibition | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.89 | 0.319 | - |
tosyl-Gly-Pro-Lys-p-nitroanilide | pH 10.0, 25°C | Pseudomonas aeruginosa | |
3.4.21.89 | 0.727 | - |
Val-Leu-Lys-p-nitroanilide | pH 10.0, 25°C | Pseudomonas aeruginosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.21.89 | extracellular | - |
Pseudomonas aeruginosa | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | 26000 | - |
- |
Pseudomonas aeruginosa |
3.4.21.89 | 26380 | - |
mass spectrometry | Pseudomonas aeruginosa |
3.4.21.89 | 30000 | - |
gel filtration, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.89 | signal peptides from preproteins + H2O | Pseudomonas aeruginosa | - |
mature proteins | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.89 | Pseudomonas aeruginosa | - |
- |
- |
3.4.21.89 | Pseudomonas aeruginosa PA103-29 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.89 | - |
Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | additional information | - |
specific activity 471 units/mg, 1 unit is DELTA A/min x total assay volume/sample volume x E 410 nm x light path (cm) where the extinction coefficient E of the product p-nitroanilide at 410 nm is 9.75 and the light path is 0.53 cm | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.89 | complement component C1q + H2O | partially degraded | Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | Fibrinogen + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | Fibrinogen + H2O | - |
Pseudomonas aeruginosa PA103-29 | ? | - |
? | |
3.4.21.89 | IgG + H2O | partially degraded | Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | Immunoglobulin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | Immunoglobulin + H2O | - |
Pseudomonas aeruginosa PA103-29 | ? | - |
? | |
3.4.21.89 | additional information | leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates | Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | additional information | leucine-4-nitroanilide, carbobenzoxy-L-phenylalanyl-L-leucyl-L-alpha-glutamyl-4-nitroanilide, Z-Val-Gly-Arg-4-nitroanilide, benzoyl-beta-alanyl-glycyl-arginine-4-nitroanilide, tosyl-glycyl-prolyl-arginine-4-nitroanilide, and L-Lys-4-nitroanilide are no substrates | Pseudomonas aeruginosa PA103-29 | ? | - |
? | |
3.4.21.89 | plasmin + H2O | partially degraded | Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | plasmin + H2O | partially degraded | Pseudomonas aeruginosa PA103-29 | ? | - |
? | |
3.4.21.89 | plasminogen + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
3.4.21.89 | signal peptides from preproteins + H2O | - |
Pseudomonas aeruginosa | mature proteins | - |
? | |
3.4.21.89 | tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | chromozym PL | Pseudomonas aeruginosa | tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? | |
3.4.21.89 | Val-Leu-Lys-p-nitroanilide + H2O | chromozym PL | Pseudomonas aeruginosa | Val-Leu-Lys + p-nitroaniline | - |
? | |
3.4.21.89 | Val-Leu-Lys-p-nitroanilide + H2O | chromozym PL | Pseudomonas aeruginosa PA103-29 | Val-Leu-Lys + p-nitroaniline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.89 | monomer | 1 * 30000, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.89 | protease IV | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | 45 | - |
- |
Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | 60 | 80 | 90% of the enzyme activity is abolished by incubation at 60°C for 40 min or 80°C for 1 min | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | 10 | - |
- |
Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.89 | 4 | 11 | activity increases from pH 4.0-9.0, 85% of maximal activity at pH 11.0, no activity above pH 12.0 | Pseudomonas aeruginosa |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.21.89 | Pseudomonas aeruginosa | isoelectric focusing | - |
8.7 |