Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, J.M.; Dando, P.M.; Rawlings, N.D.; Brown, M.A.; Young, N.E.; Stevens, R.A.; Hewitt, E.; Watts, C.; Barrett, A.J.
    Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase (1997), J. Biol. Chem., 272, 8090-8098.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 DNA sequence determination and analysis Homo sapiens
3.4.22.34 partial DNA sequence determination Sus scrofa

General Stability

EC Number General Stability Organism
3.4.22.34 enzyme tends to be absorbed to any solid material at low salt concentration Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 Human cystatin C
-
 Sus scrofa
3.4.22.34 iodoacetamide
-
 Sus scrofa
3.4.22.34 iodoacetate
-
 Sus scrofa
3.4.22.34 additional information no inhibition by E-64, i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane, no inhibition by PMSF, pepstatin and 1,10-phenanthroline Sus scrofa
3.4.22.34 N-ethylmaleimide
-
 Sus scrofa
3.4.22.34 N-Phenylmaleimide
-
 Sus scrofa
3.4.22.34 ovocystatin from chicken egg white Sus scrofa
3.4.22.34 p-chloromercuribenzoate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.34 0.05
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.8, 30°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 31000
-
 x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa
3.4.22.34 34000
-
 x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 protein + H2O Sus scrofa
-
 peptides
-
 ?
3.4.22.34 protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens Q99538
-
-
3.4.22.34 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.34 glycoprotein 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 Sus scrofa
3.4.22.34 glycoprotein 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 2200fold from kidney, to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 kidney
-
 Sus scrofa
-
3.4.22.34 liver
-
 Sus scrofa
-
3.4.22.34 additional information no activity in blood leukocytes Homo sapiens
-
3.4.22.34 placenta low activity Homo sapiens
-
3.4.22.34 spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 8.1
-
 purified enzyme, substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide, pH 5.8, 30°C Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.4.22.34 4°C to -20°C, pH 5.8, 0.05 M sodium citrate, 0.2 M NaCl, 1 mM EDTA, 10 mm cysteine, 0.1% CHAPS, stable for several months with little loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 allatotropin + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 azocasein + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 benzoyl-Asn-4-nitroanilide + H2O
-
 Sus scrofa benzoyl-Asn + 4-nitroaniline
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 bombesin + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 chicken vasoactive intestinal peptide fragment 16-28 + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 human vasoactive intestinal peptide fragment 1-12 + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 additional information no activity with angiotensin I, [Asn1,Val5]angiotensin II, peptides ASTTNYT and GSNKGAIIGLM, substance P, and dynorphin A fragment 1-13, no activity with Asn-4-methylcoumarin-7-amide or Asp-4-methylcoumarin-7-amide Sus scrofa ?
-
 ?
3.4.22.34 neurotensin + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 neurotensin fragment 1-11 + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 pig vasoactive intestinal peptide fragment 1-28 + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.34 protein + H2O
-
 Sus scrofa peptides
-
 ?
3.4.22.34 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.22.34 recombinant C-fragment of tetanus toxoid + H2O cleavage of 3 peptide bonds Sus scrofa ?
-
 ?
3.4.22.34 somatostatin + H2O
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 asparaginyl endopepidase
-
 Sus scrofa
3.4.22.34 Asparaginyl endopeptidase
-
 Homo sapiens
3.4.22.34 More belongs to the peptidase family C13 Sus scrofa
3.4.22.34 More belongs to the peptidase family C13 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.34 46
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.8, 30°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.8
-
 at 30°C Sus scrofa
3.4.22.34 6.4
-
 at 25°C Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.34 3 6
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.34 0.000005
-
 Human cystatin C below, pH 5.8, 30°C Sus scrofa